PROTEINS INVOLVED IN THE DEFENSE MECHANISMS OF HORSE SHOE CRABS

参与马蹄蟹防御机制的蛋白质

• 批准号: 3792397
• 负责人: Y DEVRIES
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792397
• 关键词: affinity chromatography; agglutinins; animal extract; antibacterial agents; bactericidal immunity; binding proteins; blood coagulation; endotoxins; horseshoe crabs; ion exchange chromatography; lectin; protein purification

Septicemia is a leading cause of morbidity and mortality among hospitalized patients. Despite the use of potent antibiotics and the newly produced monoclonal antibody against LPS, the mortality remains high. In the current studies we have isolated and characterized a number of proteins with agglutination, adhesion and antibacterial activities from the serum and the amebocytes of horseshoe crabs. The purification procedures using ion-exchange and affinity column chromatography resulted in a 55 kDa protein, limunectin, with the ability to bind to Limulus blood cells, to both gram positive and gram negative bacteria and to extracellular matrix. We have also identified a 12 kDa protein with unusual dual functional properties; it binds to endotoxin and also contains protease inhibitory activities. Coagulogen, the substrate for the clot formation, also has agglutinating activities once it is subjected to a limited proteolysis. We are in the process of studying the nature and specificity of such interactions and exploring the lectin properties of these and other components isolated from Limulus blood. Enzymes involved in the clotting cascade are also being investigated.

败血症是一个主要的发病率和死亡率的原因， 住院病人。 尽管使用了强效抗生素， 新产生的抗LPS的单克隆抗体，死亡率仍然 高 在目前的研究中，我们已经分离和表征了一种 具有凝集、粘附和抗菌作用的蛋白质数量 鲎血清和阿米巴细胞的活性。 的 使用离子交换和亲和柱的纯化方法 层析产生55 kDa的蛋白质，即免疫球蛋白， 结合鲎血细胞的能力，结合革兰氏阳性和革兰氏阳性 阴性细菌和细胞外基质。 我们还确定 一种12 kDa的蛋白质，具有不寻常的双重功能特性;它与 内毒素，还含有蛋白酶抑制活性。 凝血酶原， 凝块形成的基质也具有凝集活性 一旦它受到有限的蛋白水解。 我们正在 研究这种相互作用的性质和特殊性， 这些和其它分离组分的凝集素性质 鲎血 参与凝血级联反应的酶也被 研究了