ENDOTOXIN BINDING PROTEIN OF LIMULUS AMEBOCYTE

鲎变形细胞的内毒素结合蛋白

• 批准号: 3804682
• 负责人: Y DEVRIES
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804682
• 关键词: affinity chromatography; agglutinins; antibacterial agents; bactericidal immunity; binding proteins; endotoxins; gram negative bacteria; gram positive bacteria; horseshoe crabs; lipopolysaccharides; microorganism hemagglutinin; protein purification

The defense mechanism of Limulus Polyphemus against invading microorganisms has been one of the main interests of our laboratory. In the current studies we have isolated a number of proteins and peptides with antibacterial and/or with agglutinating properties. The purification procedures using affinity chromatography with surface components of bacteria such as lipopolysaccharide, resulted in the isolation and characterization of a 12 kD protein which has the ability to bind to Limulus blood cells and to both gram positive and gram negative bacteria. We have been studying the nature and specificity of such interactions and exploring the lectin properties of this and other components isolated from Limulus hemolymph. Partial sequence data of other agglutinins has been obtained and the full characterization of these proteins is the object of our present investigations.

灯盏细辛对入侵微生物的防御机制 一直是我们实验室的主要兴趣之一。在当前 通过研究，我们已经分离出了许多蛋白质和多肽 抗菌和/或具有凝集特性。提纯 使用亲和层析与表面组分的程序 细菌，如脂多糖，导致分离和 一种具有结合能力的12kD蛋白的鉴定 对血细胞和革兰氏阳性和革兰氏阴性细菌均有抗菌作用。 我们一直在研究这种相互作用的性质和特殊性，并 探索这一成分和从其分离的其他成分的凝集素性质 蛙血淋巴。其他凝集素的部分序列数据已经被 获得并充分表征这些蛋白质是本研究的目标 我们目前的调查。