MONOCLONAL ANTIBODIES TO HUMAN FACTOR RECEPTORS

人类因子受体单克隆抗体

• 批准号: 3794983
• 负责人: GORDON H SATO
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3794983
• 关键词: antireceptor antibody; cell growth regulation; epidermal growth factor; growth factor; growth factor receptors; growth media; human tissue; hybridomas; insulin receptor; lymphocyte; monoclonal antibody; neoplastic cell; neoplastic growth; peptide hormone; tissue /cell culture; transferrin receptor

We propose to generate human monoclonal antibodies to cell surface receptors of known functional importance by immunizing normal human peripheral blood cells in vitro under serum-free culture conditions. These antibodies will be examined for their effects on the growth of human tumor cells bearing the appropriate receptors. The methodologies that will be developed will significantly reduce the current problems involved in obtaining suitably immunized human lymphocytes and will make human monoclonal antibodies with useful specificities more accessible to the scientific community.

我们建议生成针对细胞表面的人类单克隆抗体 通过免疫正常人获得已知功能重要性的受体 外周血细胞在无血清培养条件下进行体外培养。 这些 将检查抗体对人类肿瘤生长的影响 带有适当受体的细胞。 将采用的方法论 的开发将大大减少目前涉及的问题 获得适当免疫的人类淋巴细胞并将使人类 具有有用特异性的单克隆抗体更容易获得 科学界。