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STUDIES ON PROPERTIES OF LYMPHOBLASTOID INTERFERON ALPHA COMPONENT O

STUDIES ON PROPERTIES OF LYMPHOBLASTOID INTERFERON ALPHA COMPONENT O
淋巴母细胞干扰素α组分O的性质研究
批准号:
3792458
负责人:
R Q HU
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至

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中文摘要
翻译
成分0是从人淋巴母细胞中纯化的22种成分之一
英文摘要
Component o is one of 22 components purified from human lymphoblastoid interferon-alpha (IFN-alpha) by monoclonal antibody affinity and high performance liquid chromatography. The apparent molecular weight of this component on reducing SDS-PAGE is 19,000 KD. Component o exhibits very high antiproliferative activity on Daudi and AU937 cells; the concentration that yielded 50 percent inhibition of cell growth was 0.003 ng/ml on Daudi cells and 0.05 ng/ml on AU937 cells. Binding studies using radiolabelling recombinant IFN-alpha2b indicated that component o competes very poorly for IFN-alpha2b binding to these cells. The partial amino acid sequence of component o is similar to that of the deduced amino acid sequences from IFN-alpha88 and IFN-alphaF. Western blotting analysis of component o and IFN-alpha88 with anti-IFN-alpha monoclonal antibodies revealed that their immunoreactivities are different. Component o bound to 4F2 antibody but not to NK2 and III/21 antibodies. IFN-alpha88 bound to NK2 antibodies but not to the III-21 antibody. Cross-linking experiments using Daudi cells revealed a 134-kD complex with component o.
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RELATION OF STRUCTURE AND FUNCTION OF HU IFN-GAMMA BY SITE-DIRECTED MUTAGENESIS
  • 批准号:
    3804757
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    R Q HU
  • 依托单位:
    --
CDNA CLONING AND EXPRESSION OF HUMAN IFN-ALPHA SPECIES O
  • 批准号:
    3804756
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    R Q HU
  • 依托单位:
    --
EVIDENCE FOR MULTIPLE BINDING SITES FOR SEVERAL SPECIES OF HU IFN ALPHA
  • 批准号:
    3811196
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    R Q HU
  • 依托单位:
    --
CDNA CLONING AND EXPRESSION OF HUMAN IFN-ALPHA SPECIES O AND F
  • 批准号:
    3792470
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    R Q HU
  • 依托单位:
    --
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