STUDIES ON PROPERTIES OF LYMPHOBLASTOID INTERFERON ALPHA COMPONENT O

淋巴母细胞干扰素α组分O的性质研究

• 批准号: 3792458
• 负责人: R Q HU
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792458
• 关键词: interferons; lymphocyte proliferation; protein structure function; tissue /cell culture

Component o is one of 22 components purified from human lymphoblastoid interferon-alpha (IFN-alpha) by monoclonal antibody affinity and high performance liquid chromatography. The apparent molecular weight of this component on reducing SDS-PAGE is 19,000 KD. Component o exhibits very high antiproliferative activity on Daudi and AU937 cells; the concentration that yielded 50 percent inhibition of cell growth was 0.003 ng/ml on Daudi cells and 0.05 ng/ml on AU937 cells. Binding studies using radiolabelling recombinant IFN-alpha2b indicated that component o competes very poorly for IFN-alpha2b binding to these cells. The partial amino acid sequence of component o is similar to that of the deduced amino acid sequences from IFN-alpha88 and IFN-alphaF. Western blotting analysis of component o and IFN-alpha88 with anti-IFN-alpha monoclonal antibodies revealed that their immunoreactivities are different. Component o bound to 4F2 antibody but not to NK2 and III/21 antibodies. IFN-alpha88 bound to NK2 antibodies but not to the III-21 antibody. Cross-linking experiments using Daudi cells revealed a 134-kD complex with component o.

组分o是从人淋巴母细胞中提纯的22种组分之一。 干扰素-α(干扰素-α)受单抗亲和力高 高效液相色谱。它的表观分子量 还原SDS-PAGE上的组分为19,000 kD。组份o表现得非常好 对Daudi和AU937细胞具有较高的抗增殖活性； 对细胞生长抑制50%的浓度为0.003 对Daudi细胞为0.05 ng/ml，对AU937细胞为0.05 ng/ml。约束性研究 利用放射性标记重组干扰素-α2b表明，组分o 在与这些细胞的干扰素-α2b结合方面竞争非常弱。不完整的 组份o的氨基酸序列与推导的氨基酸序列相似。 干扰素-α88和干扰素-αF的氨基酸序列。WESTERNBLOTTING 用抗干扰素-α单抗分析O组分和干扰素-α88 抗体显示，它们的免疫反应性是不同的。 组分O与4F2抗体结合，但不与NK2和III/21抗体结合。 干扰素-α88可与NK2抗体结合，但不与III-21抗体结合。 用Daudi细胞进行的交联实验显示了一个134kD的复合体 具有组件o的。