CYTOMETRY LYMPHOCYTE PROLIFERATION TEST TO DETECT BERYLLIUM HYPERSENSITIVITY

慢性乳腺炎是由免疫反应引起的， 吸入铍，发生在1%至5%的铍工人 人口 CBD的一个重要特征是对 铍。 通常在体外测定小檗碱超敏反应 使用从外周血样品分离的淋巴细胞和胸苷 合并。 我们已经开发了一种流式细胞术测定， 对激发应答的血液淋巴细胞增殖的分析 可溶性铍 已使用外围设备验证了该测试 血液样本来自被诊断患有慢性乳腺炎的患者。 最近，我们将细胞周期分析与 在相同的检测中进行免疫表型分析。 协同 环境安全和健康司，免疫流动淋巴细胞 应用扩散试验筛选LANL铍 工人队列和其他铍工人队列在DOE系统中， 对铍过敏。

Chronic Beryllium Disease is caused by an immune response to inhaled beryllium, and occurs in 1 to 5% of the beryllium worker population. A critical feature of CBD is hypersensitivity to beryllium. Beryllium hypersensitivity is typically assayed in vitro using lymphocytes isolated from peripheral blood samples and thymidine incorporation. We have developed a flow cytometry assay for the analysis of blood lymphocyte proliferation in response to a challenge with soluble beryllium. The test has been validated using peripheral blood samples from patients diagnosed with Chronic Beryllium Disease. More recently, we have combined cell cycle analysis with immunophenotype in the same assay. In collaboration with Environmental Safety and Health Division, the Immuno-Flow Lymphocyte Proliferation Test is being applied to screen the LANL beryllium worker cohort and other berylliul worker cohorts in the DOE system for hypersensitivity to beryllium.