CYTOMETRY LYMPHOCYTE PROLIFERATION TEST TO DETECT BERYLLIUM HYPERSENSITIVITY

慢性铍病是由免疫反应引起的 吸入铍，发生在1%至5%的铍工人中 人口。CBD的一个关键特征是对 铍。铍超敏反应通常在体外进行检测。 使用从外周血样本中分离的淋巴细胞和胸腺嘧啶核苷 成立为法团。我们已经开发了一种流式细胞术分析方法，用于 一次挑战反应中的血淋巴细胞增殖反应分析 用可溶的铍。该测试已使用外围设备进行了验证 慢性铍病患者的血液样本。 最近，我们将细胞周期分析与 免疫表型相同。与 环境安全与健康司，免疫流动淋巴细胞部 正在应用增殖试验对LANL铍进行筛选 能源部系统中的工人队列和其他铍工人队列 对铍过敏。

Chronic Beryllium Disease is caused by an immune response to inhaled beryllium, and occurs in 1 to 5% of the beryllium worker population. A critical feature of CBD is hypersensitivity to beryllium. Beryllium hypersensitivity is typically assayed in vitro using lymphocytes isolated from peripheral blood samples and thymidine incorporation. We have developed a flow cytometry assay for the analysis of blood lymphocyte proliferation in response to a challenge with soluble beryllium. The test has been validated using peripheral blood samples from patients diagnosed with Chronic Beryllium Disease. More recently, we have combined cell cycle analysis with immunophenotype in the same assay. In collaboration with Environmental Safety and Health Division, the Immuno-Flow Lymphocyte Proliferation Test is being applied to screen the LANL beryllium worker cohort and other berylliul worker cohorts in the DOE system for hypersensitivity to beryllium.