人 IFN-α 物种 O 的 CDNA 克隆和表达-猫眼课题宝

人 IFN-α 物种 O 的 CDNA 克隆和表达

批准号：

3804756

负责人：

R Q HU

金额：

$0.0万

依托单位：

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3804756

关键词：

computer assisted sequence analysis gene expression genetic library human genetic material tag interferons lymphocyte proliferation molecular cloning molecular site nucleic acid probes nucleic acid structure protein structure function restriction mapping transfection transposon /insertion element

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英文摘要

Twenty-two species of Hu IFN-alpha were derived from sendai virus induced Namalwa cells. We found that species O exhibits the highest antiproliferative activity on Au937 and Daudi cells but do not compete well for IFN-alpha2b binding sites. In order to further study species O, we are using IFN-alpha2 cDNA and species O specific oligonucleotides as probes to screen the Namalwa cDNA library. Once the cDNA clones coding IFN-alpha species O are found, restriction map and sequence analysis will be used to characterize the positive clones. Computer analysis of the sequence data may provide insight into the structural base of functional differences among these species. The cDNA for species O will be recloned into an appropriate expression vector and the expressed proteins will be purified and characterized.

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