EFFECT OF ANTIBODY-COMPLEMENT MEDIATED LYSIS OF HIV-2 INFECTED CELLS

抗体补体介导的 HIV-2 感染细胞裂解作用

• 批准号: 3792603
• 负责人: M P SELVAM
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792603
• 关键词: HIV infections; antiviral antibody; complement pathway; cytolysis; flow cytometry; human immunodeficiency virus 2; human tissue; virus antigen

Anti-HIV-1 positive antisera were tested for their ability to lyse HIV-1 infected cells in the presence of complement. We will test a panel of anti- HIV-2 positive human sera for their ability to activate human complement by the classical pathway using HIV-2 infected cells. Activation will be assessed by the flow cytometric detection of cell surface C3 deposition using C3-IgG conjugated to FITC. Analysis of complement-mediated cytolysis of infected cells using defined antisera against recombinant HIV-1 env or core antigens suggested that the env gp160/120 and p24 (gag) act as target antigens for antibody and complement mediated cytolysis. Cooperative effects of specific antibodies and complement inhibited HIV infection. We will compare the relative efficiencies of human positive sera and monospecific rabbit sera for their roles in complement mediated lysis of HIV-2 infected cells.

检测抗HIV-1阳性抗血清对HIV-1的裂解能力 在补体存在的情况下感染细胞。我们将测试一组 抗HIV-2阳性人血清激活人类免疫功能的研究 补体通过经典途径使用HIV-2感染细胞。 激活将通过流式细胞仪检测细胞进行评估 用FITC标记的C3-Ig G沉积表面C3。分析 利用确定的抗血清对感染细胞进行补体介导的细胞溶解 抗重组HIV-1包膜或核心抗原提示包膜 Gp160/120和p24(Gag)作为抗体的靶抗原， 补体介导的细胞溶解。特定群体的协同效应 抗体和补体可抑制HIV感染。我们将比较 人阳性血清与单特异性兔血清的相对效价 血清在补体介导的HIV-2感染的裂解中的作用 细胞。