COMPLEMENT MEDIATED KILLING OF HIV-2 INFECTED CELLS

补体介导杀死 HIV-2 感染细胞

• 批准号: 3770419
• 负责人: M P SELVAM
• 金额: --
• 依托单位: BUREAU OF HEALTH PLANNING AND RESOURCES DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3770419
• 关键词: HIV infections; HeLa cells; antibody specificity; complement pathway; cytolysis; flow cytometry; helper T lymphocyte; human immunodeficiency virus 2; serum; transfection; virus antigen

HIV-1 positive sera were tested for their ability to kill HIV-1 infected cells in the presence of complement. It was found that while fresh sera of humans didn't have antiviral activity, fresh sera of rodents showed a dose dependent viral-inactivating property against cell free HIV-1. The activity was destroyed by heating to 56 C, and apparently involves classical complement pathways. Elucidation of this mechanism may aid in understanding the lack of activity of human serum against HIV-1 and may prove useful in combined interventive strategies against HIV-1. We would like to test a panel of anti HIV-2 positive human sera for their ability to activate human complement by the classical pathway using HIV-2 infected cells. Activation will be assessed by flow cytometric detection of cell surface C3-deposition using C3-IgG conjugated to FITC. Analysis of complement mediated cytolysis of infected cells using defined antisera against recombinant HIV-1 env or core antigens suggested that the env, gp160/120 and gag, p24 acts as target antigens for antibody and complement mediated cytolysis. Complement alone reduced the spread of HIV-1 infection in CD4+ cells and the ability of HIV-1 & HIV-2 to form plaques in CD4- transfected HeLa cells. Cooperative effects of specific antibodies and complement were the most effective in inhibiting HIV-infection.

检测HIV-1阳性血清杀死HIV-1感染者的能力 在补体存在的情况下。结果发现，虽然新鲜血清的 人类没有抗病毒活性，啮齿动物的新鲜血清显示了剂量 对无细胞HIV-1的依赖性病毒灭活特性。 的 活动被加热到56 C破坏，显然涉及 经典补体途径。 阐明这一机制可能有助于 了解人血清缺乏抗HIV-1的活性， 证明在针对HIV-1的联合干预战略中是有用的。 我们 我想测试一组抗HIV-2阳性的人血清， 使用HIV-2感染通过经典途径激活人补体 细胞 将通过流式细胞术检测细胞中的 使用与FITC缀合的C3-IgG进行表面C3沉积。 分析 使用确定的抗血清的补体介导的感染细胞的细胞溶解 针对重组HIV-1 env或核心抗原的抗体表明env， gp 160/120和gag、p24作为抗体和补体的靶抗原 介导的细胞溶解。补体单独减少了HIV-1感染的传播 HIV-1和HIV-2在CD 4+细胞中形成噬斑的能力 转染HeLa细胞。 特异性抗体的协同作用， 补体在抑制HIV感染方面最有效。