CMV INDUCTION OF TUMOR NECROSIS FACTOR-ALPHA IN INFECTED TISSUE

巨细胞病毒是免疫抑制的炎症性器官疾病的主要原因 患者为了阐明巨细胞病毒引起炎症、肿瘤的机制， 研究了艾滋病合并巨细胞病毒患者的肿瘤坏死因子（TNF-α） 结肠炎采用原位杂交技术对结肠粘膜进行原位杂交 艾滋病患者和正常对照组。TNF-α阳性杂交 从患者的结肠粘膜获得mRNA的α探针， 与CMV相关，但与HIV-1感染的细胞无关。还有一个病人 CMV衍生分离物诱导原代人血单核细胞表达 TNF-α mRNA的表达，并增加分泌的TNF-α的水平。因此，CMV TNF-α的诱导可能在CMV诱导的细胞凋亡中起重要作用。 炎症此外，由于TNF-α对肿瘤细胞的已知作用， HIV-1前病毒、CMV的表达可能是激活的辅助因子 潜伏的HIV-1，而不需要细胞的共感染， 这两种病毒。

CMV is a major cause of inflammatory organ disease in immunosuppressed patients. To elucidate the mechanisms of CMV-induced inflammation, tumor necrosis factor (TNF-alpha) was studied in AIDS patients with CMV colitis. In situ hybridization techniques were used on colonic mucosa from AIDS patients and normal controls. Positive hybridization of TNF- alpha probe to mRNA was obtained with colonic mucosa from patients, and was associated with CMV, but not HIV-1 infected cells. Also, a patient derived isolate of CMV induced primary human blood monocytes to express TNF-alpha mRNA and increased the levels of secreted TNF-alpha. Thus, CMV induction of TNF-alpha may play a central role in CMV-induced inflammation. Furthermore, because of the known effects of TNF-alpha on the expression of HIV-1 provirus, CMV may be a co-factor in activation of latent HIV-1, without the requirement for co-infection of cells by the two viruses.