DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTIONS

人类巨细胞病毒感染的检测

• 批准号: 3804791
• 负责人: J F MANISCHEWITZ
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804791
• 关键词: AIDS; AIDS therapy; HIV infections; Herpesviridae disease; antiAIDS agent; antiviral agents; autologous transplantation; blood chemistry; bone marrow transplantation; clinical trials; combination chemotherapy; cytomegalovirus; drug adverse effect; early diagnosis; foscarnet; human immunodeficiency virus 1; human subject; human therapy evaluation; human tissue; interferons; interleukin 2; lung lavage; monozygotic twins; opportunistic infections; retinitis; tissue /cell culture; urinalysis; zidovudine

Since CMV is recognized as one of the leading opportunistic infections in AIDS patients, it is important to monitor blood, urine, and tissue specimens from patients enrolled in various clinical trials for the presence of CMV infections. We are currently following patients who are on combination therapies against HIV-1: AZT/IL2, IFN alpha/IL2, and a combination of AZT, IFN, AZT/IFN (three arms trials). Additionally, we are following a small group of identical twins (only one is HIV-1 infected) who have undergone autologous bone marrow transplantation for CMV infections. Another goal of this project is to determine if CMV can be isolated from bronchial lavage specimens taken from both asymptomatic as well as symptomatic (WR 1-2) HIV-1 patients. It may provide an early indicator of reactivated CMV in these patients and lead to initiation of early treatment to prevent systemic fulminating disease. Foscarnet (trisodium phosponoformate) an antiviral agent with invitro activity against both herpesviruses (including CMV) and HIV-1 was shown to have in vivo activity in AIDS patients with CMV retinitis. Improvement was documented by a reduction in isolation of CMV in culture specimens, improved vision and reduced HIV-1 p24 antigen levels.

由于巨细胞病毒被认为是一种主要的机会性感染， 对艾滋病患者来说，重要的是要监测血液、尿液和组织 来自参加各种临床试验的患者的样本， CMV感染的存在。 我们目前正在跟踪接受联合治疗的患者 对 HIV-1：AZT/IL 2、IFN α/IL 2和AZT的组合， IFN、AZT/IFN（三组试验）。 此外，我们还在跟踪一个小 一组同卵双胞胎（只有一个是HIV-1感染者）， 自体骨髓移植治疗CMV感染。 该项目的另一个目标是确定CMV是否可以从 从无症状以及 症状性（WR 1-2）HIV-1患者。 它可能提供了一个早期指标， 在这些患者中重新激活CMV，并导致早期 预防全身暴发性疾病的治疗。 膦甲酸三钠（Foscarnet）是一种体外抗病毒药物， 针对疱疹病毒（包括CMV）和HIV-1的活性已被证明， 对艾滋病患者CMV视网膜炎有体内活性。 改进 通过培养标本中CMV分离的减少来记录， 改善视力和降低HIV-1 p24抗原水平。