HHV-6 DNA INDUCED TUMORS AND TUMOR INFILTRAION LYMPHOCYTES (TIL)

我们报道了 HHV-6 DNA 转化的 NIH 3T3 细胞在 裸鼠和这些肿瘤细胞在免疫功能正常的 NIH 中具有致瘤性 瑞士老鼠。 可以从 HHV-6 基因组生成肿瘤特异性 TIL DNA诱发肿瘤。 我们最近报道了 HHV-6 亚基因组克隆 pZVH14 诱导的肿瘤细胞 (14-2T) 转移至肺部 静脉注射至免疫功能正常的小鼠体内。 TIL 也可以是 从这些 14-2T 衍生肿瘤产生，然而，这些 TIL 缺乏 肿瘤特异性溶解特性。 该项目涉及生成 由于人员短缺，TIL 将暂时停止 和适当的资源。 然而，识别和绘制地图 转化基因将在其他项目中进行。

We reported that HHV-6 DNA transformed NIH 3T3 cells produced tumors in nude mice and these tumor cells were tumorigenic in immunocompetent NIH Swiss mice. Tumor specific TIL could be generated from HHV-6 genomic DNA induced tumors. We recently reported that a HHV-6 subgenomic clone pZVH14-induced tumor cells (14-2T) metasta- sized to the lungs upon intravenous injection into immunocompetent mice. TILs could also be generated from these 14-2T derived tumors, however, these TILs lacked tumor specific lytic property. This project involving generation of TILs will be discontinued temporarily because of the lack of personnel and appropriate resources. However, identification and mapping of the transforming gene will be pursued in other projects.