HHV-6 DNA INDUCED TUMORS AND TUMOR INFILTRAION LYMPHOCYTES

最近，我们发表了HHV-6基因组和两个亚基因组， pZVH 14和pZVB 70能完全转化NIH 3 T3细胞， 转化的细胞可以在裸鼠中产生肿瘤。 我们还出版了 这些肿瘤细胞在具有免疫活性的NIH Swiss小鼠中具有致瘤性 （远系繁殖）和肿瘤特异性TIL可以从HHV-6基因组产生， DNA诱导肿瘤 然而，来自亚基因组克隆pZVH 14 DNA的TIL 诱导的肿瘤缺乏肿瘤特异性裂解能力。 瑞士老鼠 衍生的肿瘤表现出转移潜力。 进一步的研究在 确认这种转移性的进展。目前的研究也 重点是确定是否有任何HHV-6特异性抗原负责 肿瘤发生 将使用近交系动物进行肿瘤发生实验。 小鼠以产生抗原特异性TIL并鉴定肿瘤 抗原的

Recently, we have published that the HHV-6 genome and two subgenomic clones, pZVH14 and pZVB70 can fully transform NIH 3T3 cells and the transformed cells can produce tumors in nude mice. We have also published that these tumor cells are tumorigenic in immunocompetent NIH Swiss mice (outbred) and tumor specific TIL can be generated from the HHV-6 genomic DNA induced tumors. However, TIL from the subgenomic clone, pZVH14 DNA induced tumors lacked the tumor specific lytic capability. The Swiss mice derived tumors demonstrated metastatic potential. Further studies are in progress to confirm this metastatic property. Current studies are also focused on identifying if any HHV-6 specific antigen is responsible for tumorigenesis. Tumorigenesis experiments will be performed using inbred mice in order to generate antigen specific TIL and to identify the tumor antigen.