HHV-6 DNA INDUCED TUMORS AND TUMOR INFILTRAION LYMPHOCYTES

HHV-6 DNA 诱导的肿瘤和肿瘤浸润淋巴细胞

• 批准号: 3804799
• 负责人: A RAZZAQUE
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804799
• 关键词: athymic mouse; cytotoxic T lymphocyte; genome; human herpesvirus 6; laboratory mouse; metastasis; neoplastic transformation; tissue /cell culture; tumor antigens; viral carcinogenesis

Recently, we have published that the HHV-6 genome and two subgenomic clones, pZVH14 and pZVB70 can fully transform NIH 3T3 cells and the transformed cells can produce tumors in nude mice. We have also published that these tumor cells are tumorigenic in immunocompetent NIH Swiss mice (outbred) and tumor specific TIL can be generated from the HHV-6 genomic DNA induced tumors. However, TIL from the subgenomic clone, pZVH14 DNA induced tumors lacked the tumor specific lytic capability. The Swiss mice derived tumors demonstrated metastatic potential. Further studies are in progress to confirm this metastatic property. Current studies are also focused on identifying if any HHV-6 specific antigen is responsible for tumorigenesis. Tumorigenesis experiments will be performed using inbred mice in order to generate antigen specific TIL and to identify the tumor antigen.

最近，我们发表了HHV-6基因组和两个亚基因组 克隆pZVH14和pZVB70可完全转化NIH3T3细胞 转化的细胞可以在裸鼠体内产生肿瘤。我们还发布了 这些肿瘤细胞在免疫功能正常的NIH瑞士小鼠体内具有致瘤性 从HHV-6基因组中可以产生(异种)和肿瘤特异的TIL DNA诱导的肿瘤。然而，从亚基因组克隆pZVH14 DNA中提取的TIL 诱导的肿瘤缺乏肿瘤特异性的裂解能力。瑞士老鼠 衍生肿瘤显示出转移潜能。进一步的研究正在进行中 确认这种转移性质的进展。目前的研究也是 重点是确定是否有任何HHV-6特异性抗原导致 肿瘤发生学。肿瘤发生实验将使用近亲交配进行 小鼠为产生抗原特异性TIL并鉴定肿瘤 抗原。