COOPERATING ACTIVITY OF HPV-16 AND HSV-2 OF HCMV DNA IN TRANSFORMING HUMAN CELLS

人乳头瘤病毒16型（HPV-16）、单纯疱疹病毒2型 （HSV-2）和人类巨细胞病毒与宫颈癌有关。 我们研究了HSV-2和HPV-16 DNA序列是否可以在早期转化为 传代人细胞。 牙龈成纤维细胞来源于正常 个体（N-16）和来自一个 用苯妥英治疗的癫痫患者（R-30）转染 HPV-16 DNA单独或与HSV-2 DNA组合。 HPV-16 DNA 高效永生化R-30细胞，但不能永生化N-16细胞。 R-30细胞共- 转染HPV-16和HSV-2 DNA的细胞非整倍体增多， 在软琼脂中比单独用HPV-16 DNA转染的R-30更好。 两 用Southern blot和PCR检测HSV-2和HPV-16 DNA序列 转化细胞中的分析。 我们发表了一篇论文， 数据 我们将检测HPV E6和E7基因的表达， 这些转化体中细胞癌基因的激活。 我们还 我想用HCMV DNA序列进行类似的研究。 这些数据 提示HPV-16和HSV-2DNA序列可在多步骤中协同作用 转变的过程。

Human papilloma virus type 16 (HPV-16), herpes simplex virus type 2 (HSV-2) and human cytomegalovirus have been linked to cervical cancer. We examined whether HSV-2 and HPV-16 DNA sequences can transform early passage human cells. Gingival fibroblasts derived from a normal individual (N-16) and from a hyperplastic gingival tissue of an epileptic patient (R-30) treated with phenytoin were transfected with HPV-16 DNA alone or in combination with HSV-2 DNA. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells co- transfected with HPV-16 and HSV-2 DNAs were more aneuploid and grew better in soft agar than R-30 transfected with HPV-16 DNA alone. Both HSV-2 and HPV-16 DNA sequences were detected by Southern blot and PCR analysis in transformed cells. We have published a paper with these data. We will examine the expression of E6 and E7 genes of HPV and activation of cellular oncogenes in these transformants. We would also like to pursue similar studies with HCMV DNA sequences. These data suggest that HPV-16 and HSV-2 DNA sequences can cooperate in multistep process of transformation.