COOPERATING ACTIVITY OF HPV-16 AND HSV-2 OF HCMV DNA IN TRANSFORMING HUMAN CELLS

HCMV DNA 的 HPV-16 和 HSV-2 在转化人类细胞中的协同活性

• 批准号: 3792531
• 负责人: A RAZZAQUE
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792531
• 关键词: aneuploidy; cell line; cell transformation; cytomegalovirus; fibroblasts; gene expression; gingiva; herpes simplex virus 2; human papillomavirus; human tissue; nucleic acid sequence; oncogenic virus; phenytoin; polymerase chain reaction; southern blotting; transfection; transforming virus; virus DNA; virus related neoplasm /cancer; virus replication

Human papilloma virus type 16 (HPV-16), herpes simplex virus type 2 (HSV-2) and human cytomegalovirus have been linked to cervical cancer. We examined whether HSV-2 and HPV-16 DNA sequences can transform early passage human cells. Gingival fibroblasts derived from a normal individual (N-16) and from a hyperplastic gingival tissue of an epileptic patient (R-30) treated with phenytoin were transfected with HPV-16 DNA alone or in combination with HSV-2 DNA. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells co- transfected with HPV-16 and HSV-2 DNAs were more aneuploid and grew better in soft agar than R-30 transfected with HPV-16 DNA alone. Both HSV-2 and HPV-16 DNA sequences were detected by Southern blot and PCR analysis in transformed cells. We have published a paper with these data. We will examine the expression of E6 and E7 genes of HPV and activation of cellular oncogenes in these transformants. We would also like to pursue similar studies with HCMV DNA sequences. These data suggest that HPV-16 and HSV-2 DNA sequences can cooperate in multistep process of transformation.

人乳头瘤病毒 16 型 (HPV-16)、单纯疱疹病毒 2 型 (HSV-2) 和人类巨细胞病毒与宫颈癌有关。 我们检查了 HSV-2 和 HPV-16 DNA 序列是否可以早期转化 传代人体细胞。 牙龈成纤维细胞来源于正常牙龈 个体（N-16）和来自增生性牙龈组织 用苯妥英治疗的癫痫患者（R-30）被转染 单独的 HPV-16 DNA 或与 HSV-2 DNA 组合。 HPV-16 DNA 有效地使 R-30 细胞永生化，但不能使 N-16 细胞永生化。 R-30细胞共- 转染 HPV-16 和 HSV-2 DNA 的细胞非整倍体较多且生长更快 在软琼脂中比单独用 HPV-16 DNA 转染的 R-30 效果更好。 两个都 通过 Southern blot 和 PCR 检测 HSV-2 和 HPV-16 DNA 序列 转化细胞中的分析。 我们已经发表了一篇关于这些的论文 数据。 我们将检查 HPV E6 和 E7 基因的表达， 这些转化体中细胞癌基因的激活。 我们也会 喜欢对 HCMV DNA 序列进行类似的研究。 这些数据 表明 HPV-16 和 HSV-2 DNA 序列可以多步合作 转变的过程。