PERSISTENT HIV-1 INFECTION OF HUMAN MAMMARY EPITHELIAL CELLS

我们正在研究从正常乳房和乳房分离出的细胞系。 癌确定乳腺上皮细胞(MES)是否可以 感染了HIV-1病毒。MES是HIV的潜在宿主，可能是一种 在血清阳性母亲的母乳中检测到艾滋病毒的来源。 用HIV-1(MN)感染6种不同的ME细胞系(以及H9 用于比较的细胞)，它们被p24监控病毒的产生 抗原检测、DNA聚合酶链式反应分析和免疫荧光检测。这些被感染的 培养已经保存了60多天，并在 文化。P24检测和DNA聚合酶链式反应均可检测到低水平感染。 由p24抗原测定的感染时间进程不同于 不同的细胞系。实验正在进行中，以确定 这些细胞是否正在产生传染性病毒，并确定 细胞和/或细胞因子可能参与维持一种 潜伏的艾滋病毒感染。

We are studying cell lines isolated from both normal breast and breast carcinomas to determine whether mammary epithelial cells (MEs) can be infected by HIV-1. MEs are a potential reservoir for HIV and may be a source of the HIV detected in the breast milk of seropositive mothers. HIV-1 (MN) was used to infect six different ME cell lines (as well as H9 cells for comparison), which were monitored for virus production by p24 antigen assay, DNA PCR analysis and immunofluorescence. These infected cultures have been maintained for over 60 days and are continuing in culture. Low level infection is detectable by p24 assay and by DNA PCR. The time course of infection as measured by p24 antigen varied among different cell lines. Experiments are in progress to 1) ascertain whether these cells are producing infectious virus and to 2) identify the cells and/or cellular factors which may be involved in maintaining a latent HIV infection.