INFECTION OF RABBITS WITH HUMAN IMMUNODEFICIENCY VIRUS 1

人类免疫缺陷病毒 1 感染兔子

• 批准号: 3860004
• 负责人: H KULAGA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3860004
• 关键词: AIDS; HIV infections; antiviral antibody; communicable disease transmission; human immunodeficiency virus 1; immunosuppression; laboratory rabbit; polymerase chain reaction; serology /serodiagnosis; virus DNA

Injection of rabbits with human T-cell line that has been infected with HIV-1 causes seroconversion within 4 weeks of injection. Normally, 5 X 10 6 cells are administered. If higher doses are used, the seroconversion occurs earlier and the levels of antibody are elevated. One hundred and thirty rabbits have been successfully infected. Five percent of the animals did not seroconvert after the original dose of infected cells, but antibody could be detected following a booster injection. The virus has been detected by polymerase chain reaction amplification of viral DNA and RNA in brain as well as lymphoid tissues. Immune responses to tetanus toxoid and to BCG have been monitored in infected animals and were shown to be diminished or absent.

用已经感染了人T细胞系的兔注射 HIV-1在注射后4周内引起血清转化。 通常，5 X 10 给予6个细胞。 如果使用更高剂量， 发生得更早，抗体水平也会升高。 130只兔子被成功感染。 五 %的动物在初始剂量后未发生血清转化 感染细胞，但在加强免疫后可以检测到抗体 注射 该病毒已通过聚合酶链反应检测出来 大脑和淋巴组织中病毒DNA和RNA的扩增。 对破伤风类毒素和卡介苗的免疫反应已在2000年进行了监测， 感染的动物，并显示减少或不存在。