ANTIBODIES TO HUMAN IFN IN J.L.P. PATIENTS TREATED WITH HUMAN IFN

人血清白蛋白治疗患者660份血清标本 白细胞α干扰素(胡干扰素(Le))(纽约血液中心，梅尔维尔， (纽约州)青少年喉乳头状瘤病(J.L.P.)进行了测试，以检测 存在上述α-抗体的中和抗体 干扰素。11个样本被发现含有中和抗体。 从纽约不列颠哥伦比亚州的阿尔法干扰素。其中10个含有中和 人类白细胞干扰素抗体(Alferon；Interferon Science， 新泽西州新不伦瑞克)其中9株含有中和抗体 重组干扰素(Intron A，先灵葆雅，新泽西州凯尼尔沃斯)。 初步数据显示，这11个中和阳性样本 在酶联免疫吸附试验中均呈阳性，与相同类型的 它们中和了干扰素，当然，这些检测必须是 重复一遍。 同样令人感兴趣的是，是否有任何阳性样本与 任何人类淋巴母细胞成分都是在我们的 实验室。将使用洗脱液进行中和分析 从从中提纯这些成分的单抗柱子中提取。 如果看到任何中和作用，在该物质中发现的单独成分 将对洗脱液进行检测。

Six hundred and sixty serum samples from 135 patients treated with human leukocyte alpha interferon (Hu IFN (Le) (New York Blood Center, Melville, N.Y.) for Juvenile Laryngeal Papillomatosis (J.L.P.) were tested for the presence of neutralizing antibodies to the above-mentioned alpha interferon. Eleven samples were found to contain neutralizing antibodies to alpha interferon from the N.Y.B.C. Ten of these contained neutralizing antibodies to human leukocyte ` interferon (Alferon; Interferon Sciences, New Brunswick, N.J.) and nine contained neutralizing antibodies to recombinant ` interferon (Intron A, Schering-Plough, Kenilworth, N.J.). Preliminary data suggest that the eleven neutralization positive samples are all positive in ELISA assays for binding to the same types of interferon which they neutralize but, of course, these assays must be repeated. Also of interest is whether any of the positive samples cross react with any of the human lymphoblastoid components which are purified in our laboratory. Neutralization assays will be performed using the eluates from monoclonal antibody columns from which these components are purified. If any neutralization is seen, the individual components found in that eluate will be assayed.