ANTIBODIES TO HUMAN IFN IN JPL PATIENTS TREATED WITH HUMAN IFN

接受人干扰素治疗的 JPL 患者中的人干扰素抗体

• 批准号: 3748188
• 负责人: J BEKISZ
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3748188
• 关键词: adolescence (12-20); antigen antibody reaction; biological response modifiers; cutaneous papilloma; enzyme linked immunosorbent assay; human therapy evaluation; human tissue; hyperplasia; immunotherapy; interferon alpha; larynx disorder; larynx neoplasms; neoplasm /cancer immunotherapy; neutralizing antibody; recombinant proteins

Serum samples from 135 patients treated with human leukocyte alpha interferon (Hu IFN (Le) (New York Blood Center, Melville, N.Y. ) for Juvenile Laryngeal Papillomatosis (J.L.P.) were tested for the presence of neutralizing antibodies to the above mentioned alpha interferon. Eleven samples were found to contain neutralizing antibodies to alpha interferon from the N.Y.B.C. Ten of these contained neutralizing antibodies to Human leukocyte alpha interferon (Alferon; Interferon Sciences, New Brunswick, N.J.) and nine contained neutralizing antibodies to recombinant alpha interferon (Intron A, Schering-Plough, Kenilworth, N.J.). None of the samples were seen to bind to either Alferon or Intron in ELISA assays. Six, however, did bind to Wellferon (Burroughs Wellcome, Beckenham, U.K.) which is a natural lymphoblastoid interferon. Since we purify IFN-a components in our laboratory using Wellferon as starting material, we were interested to determine if there was any binding of the positive samples to our purified components. We found a low level of binding by three of the samples to an eluate which contains two of the species. Although the quantity of the aforementioned species is a limiting factor, we are in the process of running assays which will show if the antibody present reacts with one or both of these components.

来自135例接受人白细胞α治疗的患者的血清样本 干扰素（Hu IFN（Le））（纽约血液中心，梅尔维尔，纽约。）的 幼年喉乳头状瘤病（J. L. P.）进行了检测 对上述α干扰素的中和抗体。 11个样本被发现含有α中和抗体 其中10种含有中和性的干扰素， 抗人白细胞α干扰素抗体 科学，新玩法，新泽西州）九个含有中和抗体 重组α干扰素（Intron A，Schering-Plough，Kenilworth， N.J.）。 没有观察到样品与Alferon或Intron结合 在ELISA测定中。 六，然而，没有绑定到Wellferon（巴勒斯 Wellcome，贝克纳姆，英国）其是天然的类淋巴母细胞干扰素。 由于我们在我们的实验室中使用Wellferon纯化IFN-α组分， 作为起始材料，我们有兴趣确定是否有任何 阳性样品与我们纯化的组分的结合。 我们发现了一个 三个样品与含有以下物质的酶的结合水平较低 两个物种。 虽然上述物种的数量 是一个限制因素，我们正在进行检测， 显示抗体是否与其中一种或两种反应 件.