GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
批准号:
3796482
负责人:
M M GOTTESMAN
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
CHO cells Escherichia coli Pseudomonas cell growth regulation complementary DNA cyclic AMP cytogenetics drug resistance electroporation enzyme activity enzyme complex fibroblasts gel electrophoresis gene expression gene mutation genetic library genetic regulation genetic transcription messenger RNA molecular cloning mutant neoplasm /cancer genetics nucleic acid sequence phosphodiesterases protein kinase protein kinase A tissue /cell culture transforming growth factors transposon /insertion element yeasts
中文摘要
中国仓鼠卵巢(CHO)成纤维细胞是一个很好的模型系统
用于研究某些方面的遗传学和生物化学,
培养细胞的行为。 我们用这个系统来分析
环腺苷酸调节细胞生长和基因表达的方式,
哺乳动物细胞 CHO细胞的cAMP作用机制已被证实。
通过产生对cAMP的生长抑制具有抗性的细胞系来研究。
产生对cAMP抗性的一种方式是通过以下突变:
cAMP依赖性蛋白激酶,其导致改变的调节
(RI)或催化(C)亚基。 cAMP依赖性蛋白激酶缺陷
阻断cAMP刺激的转录并减少mRNA的量,
CHO细胞和肾上腺Y-1细胞中的多药物转运蛋白。 我们有
构建了CHO RI亚基的几种突变形式和突变C
亚基并在大肠杆菌中表达。细菌表达的突变RI
影响C识别RI和cAMP与RI结合的位点
在与C亚基的结合和cAMP刺激的
RI和C亚基的解离。 一种改进的系统,
哺乳动物细胞中cAMP依赖性蛋白激酶的失活已经被
开发 将CHO RI cDNA克隆到一个
进行哺乳动物表达系统以创建RI突变体文库,
可以电穿孔到CHO细胞中,然后选择显性的
阴性突变体 这个矢量系统有望用于分析
cAMP在哺乳动物细胞中的作用。
英文摘要
The Chinese hamster ovary (CHO) fibroblast is an excellent model system
for the study of the genetics and biochemistry of some aspects of the
behavior of cultured cells. We are using this system to analyze the
manner in which cyclic AMP regulates cell growth and gene expression in
mammalian cells. The mechanism of cAMP action of CHO cells has been
studied by generating cell lines resistant to growth inhibition by cAMP.
One way to develop resistance to cAMP is through mutations in
cAMP-dependent protein kinases which result in either altered regulatory
(RI) or catalytic (C) subunits. Defects in cAMP-dependent protein kinase
block cAMP-stimulated transcription and reduce amounts of mRNA for the
multidrug transporter in CHO cells and in adrenal Y-1 cells. We have
constructed several mutant forms of the CHO RI subunit and a mutant C
subunit and expressed these in E.coli. Bacterially expressed mutant RI's
affecting sites for recognition of RI by C and for binding of cAMP to RI
have defects in associating with the C subunit and in cAMP-stimulated
dissociation of RI and C subunits. An improved system for the
inactivation of cAMP dependent protein kinase in mammalian cells has been
developed. PCR-directed mutagenesis of the CHO RI cDNA cloned into a
mammalian expression system is done to create a RI mutant library which
can be electroporated into CHO cells followed by selection of dominant
negative mutants. This vector system promises to be useful for analysis
of cAMP effects in mammalian cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
GENETIC ANALYSIS OF THE MULTIPLE DRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
-
批准号:4691877
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
CONTROL OF SYNTHESIS OF A TRANSFORMATION-DEPENDENT SECRETED GLYCOPROTEIN
-
批准号:4691868
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3752049
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3796485
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC ANALYSIS OF THE MULTIPLE DRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
-
批准号:3808546
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:3916342
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3774337
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:5200962
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:3939315
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3939320
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:3963034
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:4691862
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:3813383
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC ANALYSIS OF THE MULTIPLE DRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
-
批准号:3813393
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC AND BIOCHEMICAL ANALYSIS OF CELL BEHAVIOR
-
批准号:3774334
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC ANALYSIS OF THE MULTIPLE DRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
-
批准号:3916353
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3916345
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
GENETIC ANALYSIS OF THE MULTIPLE DRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
-
批准号:3963049
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
CONTROL OF SYNTHESIS OF A TRANSFORMATION-DEPENDENT SECRETED GLYCOPROTEIN
-
批准号:3963040
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
SYNTHESIS AND FUNCTION OF A TRANSFORMATION-DEPENDENT SECRETED LYSOSOMAL PROTEASE
-
批准号:3813385
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:M M GOTTESMAN
-
依托单位:
国内基金
海外基金
登录
查看更多内容
asr基因调控酸诱导的Escherichia coli O157:H7形成VBNC状态的机制研究
-
批准号:32302245
-
项目类别:青年科学基金项目
-
资助金额:30.00万元
-
批准年份:2023
-
负责人:潘寒姁
-
依托单位:
小肠中Escherichia coli分泌细菌毒素诱导肠屏障损伤及细菌易位在炎症性肠病中的机制研究
-
批准号:82371775
-
项目类别:面上项目
-
资助金额:46万元
-
批准年份:2023
-
负责人:朱慧媛
-
依托单位:
基于Escherichia coli O157:H7亚致死态细胞探究超高压与原儿茶酸协同杀菌机制
-
批准号:31871817
-
项目类别:面上项目
-
资助金额:60.0万元
-
批准年份:2018
-
负责人:孙爱东
-
依托单位:
肠肝轴:从临床患者分离的肠道致病菌株Escherichia coli NF73-1对非酒精性脂肪性肝病的作用及机制研究
-
批准号:81873549
-
项目类别:面上项目
-
资助金额:57.0万元
-
批准年份:2018
-
负责人:刘玉兰
-
依托单位:
高压二氧化碳诱导Escherichia coli O157:H7形成VBNC状态的分子机制
-
批准号:31571933
-
项目类别:面上项目
-
资助金额:57.0万元
-
批准年份:2015
-
负责人:廖小军
-
依托单位:
超高压诱导牛肉中Escherichia coli O157:H7亚致死损伤及其修复研究
-
批准号:31371861
-
项目类别:面上项目
-
资助金额:15.0万元
-
批准年份:2013
-
负责人:江芸
-
依托单位:
高压二氧化碳诱导Escherichia coli O157:H7形成VBNC状态的机制
-
批准号:31371845
-
项目类别:面上项目
-
资助金额:15.0万元
-
批准年份:2013
-
负责人:廖小军
-
依托单位:
高密度二氧化碳致死Escherichia coli的相关蛋白质确证及其结构变化研究
-
批准号:31171774
-
项目类别:面上项目
-
资助金额:66.0万元
-
批准年份:2011
-
负责人:张德权
-
依托单位: