LAMIN PEPTIDES STIMULATE HUMAN NEUTROPHIL CHEMOKINESIS

层粘连蛋白是一种850 kDa的基底膜糖蛋白，已被报道为 刺激兔腹膜定向细胞迁移(趋化) 渗出性中性粒细胞(PMN)。人中性粒细胞表达层粘连蛋白受体， 然而，层粘连蛋白对人中性粒细胞的趋化活性仍然存在 未定义。层粘连蛋白，从Engelbreth-Holm-Spot肿瘤中分离出来，以及10 与层粘连蛋白A和层粘连蛋白不同区域相对应的合成肽 比较了B1链刺激人中性粒细胞的能力 趋化和激活的随机迁移(趋化运动)通过 48孔微趋化试验中的聚碳酸酯膜过滤器。 多肽F-9、F-11、F-12和F-13来源于层粘连蛋白B1 链跨区(J.Cell Biol.1988,107：1253)，而6个肽 均来源于层粘连蛋白A链(J.Invest.皮尔玛，1991，97：14)： 来自氨基末端顶球的多肽Tg-1、Gd1、Gd-3、 来自羧基末端球状结构域的GD-6和GD-7；以及多肽 AG-1来自羧基末端上方的球状结构域。层粘连蛋白与 在1-200微克/毫升的浓度范围内对多肽进行评估 运动性分析。仅有F-12、Tg-1、Gd-6和Gd-1四种多肽， 刺激中性粒细胞趋化运动迁移。层粘连蛋白和其他多肽 未能刺激人中性粒细胞迁移。这些结果表明， 完整的层粘连蛋白不会刺激人中性粒细胞迁移。三种多肽 来自层粘连蛋白A链的TG-1、GD-6和GD-1，以及来自 B1链，F-12，刺激化学动力迁移。这些结果表明 在此过程中可能产生的特定层粘蛋白片段 基底膜的降解可刺激人中性粒细胞 迁移。 这项研究的结果已提交给 1991年美国细胞生物学学会会议。

Laminin, an 850 kDa basement membrane glycoprotein, has been reported to stimulate directed cell migration (chemotaxis) of rabbit peritoneal exudate neutrophils (PMN). Human PMN express receptors for laminin, however, the chemoattractant activity of laminin for human PMN remains undefined. Laminin, isolated from Engelbreth-Holm-Swarm tumor, and ten synthetic peptides corresponding to various regions of the laminin A and B1 chains were compared for their abilities to stimulate human PMN chemotaxis and activated random migration (chemokinesis) through polycarbonate membrane filters in a 48-well microchemotaxis assay. Peptides F-9, F-11, F-12, and F-13 were derived from the laminin B1 chain cross-region (J. Cell Biol. 1988, 107: 1253), while six peptides were derived from the laminin A chain (J. Invest. Derm., 1991, 97:14): peptide TG-1 from the amino terminus top globule; peptides GD1, GD-3, GD-6, and GD-7 from the carboxy terminus globular domain; and peptide AG-1 from above the carboxy terminus globular domain. Laminin and the peptides were evaluated over a concentration range of 1-200 ug/mL in the motility assays. Only four of the peptides, F-12, TG-1, GD-6 and Gd-1, stimulated PMN chemokinetic migration. Laminin and the other peptides failed to stimulate human PMN migration. These results demonstrate that intact laminin does not stimulate human PMN migration. Three peptides from the laminin A chain, TG-1, GD-6 and GD-1, and one peptide from the B1 chain, F-12, stimulate chemokinetic migration. These results suggest that specific laminin fragments which may be generated during degradation of the basement membrane can stimulate human neutrophil migration. The results of this study have been submitted for presentation at the 1991 American Society for Cell Biology Meeting.