LAMIN PEPTIDES STIMULATE HUMAN NEUTROPHIL CHEMOKINESIS

层粘连蛋白是一种850kDa的基底膜糖蛋白，已被报道为 刺激兔腹膜渗出液的定向细胞迁移(趋化) 中性粒细胞(PMN)。然而，人中性粒细胞表达层粘连蛋白受体 层粘连蛋白对人PMN的趋化活性尚不明确。 层粘连蛋白，从Engelbreth-Holm-Spot肿瘤中分离出来，以及10个人工合成的 与层粘连蛋白A和B1链不同区域相对应的多肽 比较了它们刺激人中性粒细胞趋化和 聚碳酸酯膜中的活化随机迁移(化学运动) 48孔微趋化试验中的过滤器。F-9、F-11、F-12和 F-13来源于层粘连蛋白B1链交叉区(J.Cell Biol. 1988,107：1253)，而6个肽来自层粘连蛋白A链 (J.投资。Der.，1991，97：141)：来自氨基末端的多肽Tg-1 球形；羧基末端的多肽GD-1、GD-3、GD-6和GD-7 球状结构域；以及来自羧基末端上方的多肽AG-1 域。在一定的浓度范围内评估层粘连蛋白和多肽 活力测定为1-200微克/毫升。只有四种多肽，F-12， TG-1、GD-6和GD-1刺激中性粒细胞趋化迁移。层粘连蛋白和 其他多肽均不能刺激人中性粒细胞迁移。相比之下， 层粘连蛋白刺激兔外周血中中性粒细胞的化学运动。这些结果 人和兔中性粒细胞运动反应的物种差异 为了层粘连蛋白。来自层粘连蛋白A链的三个肽，TG-1，GD-6和GD-1， 而B1链上的一种多肽F-12可以刺激人PMN的化学运动 迁移。这些结果表明，特定的层粘连蛋白片段可能 在基底膜降解过程中产生的可刺激人体 中性粒细胞迁移。 这项研究的结果在1991年的美国学会发表。 细胞生物学会议，并提交作为手稿出版。

Laminin, an 850 Kda basement membrane glycoprotein, has been reported to stimulate directed cell migration (chemotaxis) of rabbit peritoneal exudate neutrophils (PMN). Human PMN express receptors for laminin, however, the chemoattractant activity of laminin for human PMN remains undefined. Laminin, isolated from Engelbreth-Holm-Swarm tumor, and ten synthetic peptides corresponding to various regions of the laminin A and B1 chains were compared for their abilities to stimulate human PMN chemotaxis and activated random migration (chemokinesis) through polycarbonate membrane filters in a 48-well microchemotaxis assay. Peptides F-9, F-11, F-12, and F-13 were derived from the laminin B1 chain cross-region (J. Cell Biol. 1988, 107:1253), while six peptides were derived from the laminin A chain (J. Invest. Derm., 1991, 97:141): peptide TG-1 from the amino terminus top globule; peptides GD-1, GD-3, GD-6, and GD-7 from the carboxy terminus globular domain; and peptide AG-1 from above the carboxy terminus globular domain. Laminin and the peptides were evaluated over a concentration range of 1-200 ug/ml in the motility assays. Only four of the peptides, F-12, TG-1, GD-6 and GD-1, stimulated PMN chemokinetic migration. Laminin and the other peptides failed to stimulate human PMN migration. In contrast, laminin stimulated rabbit peripheral blood PMN chemokinesis. These results demonstrate a species difference in human and rabbit PMN motility responses to laminin. Three peptides from the laminin A chain, TG-1, GD-6, and GD-1, and one peptide from the B1 chain, F-12, stimulate human PMN chemokinetic migration. These results suggest that specific laminin fragments which may be generated during basement membrane degradation can stimulate human neutrophil migration. The results of this study were presented at the 1991 American Society for Cell Biology Meeting and are submitted for publication as a manuscript.