LAMIN PEPTIDES STIMULATE HUMAN NEUTROPHIL CHEMOKINESIS

层粘连蛋白是一种分子量为850 Kda的基底膜糖蛋白， 兔腹腔渗出液诱导细胞趋化性 中性粒细胞（PMN）。 人PMN表达层粘连蛋白受体，然而， 层粘连蛋白对人中性粒细胞的化学引诱活性仍不清楚。 层粘连蛋白，分离自Engelbreth-Holm-Swarm肿瘤，和10种合成的 对应于层粘连蛋白A和B1链不同区域的肽 比较了它们刺激人PMN趋化性的能力， 通过聚碳酸酯膜的活化随机迁移（化学运动） 在48孔微趋化性测定中过滤。 肽F-9、F-11、F-12和 F-13衍生自层粘连蛋白B1链交叉区（J. Cell Biol. 1988，107：1253），而六种肽衍生自层粘连蛋白A链 (J. Invest.皮肤，1991，97：141）：来自氨基末端顶部的肽TG-1 小球;羧基末端的肽GD-1、GD-3、GD-6和GD-7 球状结构域;和来自球状结构域羧基末端上方的肽AG-1 域 在一定浓度范围内评价层粘连蛋白和肽 1-200 μ g/ml。 只有四种肽，F-12， TG-1、GD-6和GD-1刺激PMN的化学动力学迁移。 层粘连蛋白和 其它肽不能刺激人PMN迁移。 与此相反， 层粘连蛋白刺激兔外周血中性粒细胞趋化。 这些结果 证明人和兔PMN运动反应的种属差异 层粘连蛋白。 来自层粘连蛋白A链的三种肽，TG-1、GD-6和GD-1， B1链的一种肽F-12刺激人PMN的化学动力学 迁移 这些结果表明，特定的层粘连蛋白片段， 在基底膜降解过程中产生的， 中性粒细胞迁移。 这项研究的结果发表在1991年的美国医学会上。 细胞生物学会议，并提交作为手稿出版。

Laminin, an 850 Kda basement membrane glycoprotein, has been reported to stimulate directed cell migration (chemotaxis) of rabbit peritoneal exudate neutrophils (PMN). Human PMN express receptors for laminin, however, the chemoattractant activity of laminin for human PMN remains undefined. Laminin, isolated from Engelbreth-Holm-Swarm tumor, and ten synthetic peptides corresponding to various regions of the laminin A and B1 chains were compared for their abilities to stimulate human PMN chemotaxis and activated random migration (chemokinesis) through polycarbonate membrane filters in a 48-well microchemotaxis assay. Peptides F-9, F-11, F-12, and F-13 were derived from the laminin B1 chain cross-region (J. Cell Biol. 1988, 107:1253), while six peptides were derived from the laminin A chain (J. Invest. Derm., 1991, 97:141): peptide TG-1 from the amino terminus top globule; peptides GD-1, GD-3, GD-6, and GD-7 from the carboxy terminus globular domain; and peptide AG-1 from above the carboxy terminus globular domain. Laminin and the peptides were evaluated over a concentration range of 1-200 ug/ml in the motility assays. Only four of the peptides, F-12, TG-1, GD-6 and GD-1, stimulated PMN chemokinetic migration. Laminin and the other peptides failed to stimulate human PMN migration. In contrast, laminin stimulated rabbit peripheral blood PMN chemokinesis. These results demonstrate a species difference in human and rabbit PMN motility responses to laminin. Three peptides from the laminin A chain, TG-1, GD-6, and GD-1, and one peptide from the B1 chain, F-12, stimulate human PMN chemokinetic migration. These results suggest that specific laminin fragments which may be generated during basement membrane degradation can stimulate human neutrophil migration. The results of this study were presented at the 1991 American Society for Cell Biology Meeting and are submitted for publication as a manuscript.