GENERATION OF ANTIBODIES TO T CELL RECEPTOR

T 细胞受体抗体的产生

• 批准号: 3809945
• 负责人: Philippa C. Marrack
• 金额: --
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809945
• 关键词: T cell receptor; antibody formation; genetic manipulation; immunoglobulin structure; laboratory mouse; laboratory rabbit; laboratory rat; monoclonal antibody; tissue /cell culture

The aim of this project is to produce monoclonal antibodies against various components of the T cell receptor-T3 complex in mouse. These reagents will be used to study the assembly of the complex during development, to investigate differences in the structure of T3 on mouse alpha beta- or gamma delta-bearing cells, in blocking experiments to establish the physical relationships between different components of the complex and as probes for the analysis of the T cell repertoire in different mouse strains and different responses. They will be used by other projects in this program. It is also expected that like the other antibodies of this types that we and others have produced, the new monoclonal anti-T3 cell receptor and anti-T3 antibodies will have multiple uses by the many other investigators in this field to whom they will be given freely. We plan to produce monoclonal antibodies against murine V alphas (specifically V alpha11 and V alpha5) and V betas (specifically V beta3 and V beta5), against T cell receptor constant region determinants and against T3 proteins.

该项目的目的是生产抗 小鼠T细胞受体-T3复合物的各种组分。 这些试剂将用于研究复合物的组装 在发育过程中，为了研究 T3对小鼠α-β或γ-δ细胞的作用，阻断 建立物理关系的实验 复合物的不同组分，并作为分析的探针 在不同的小鼠品系和不同的 应答 它们将用于本计划中的其他项目。 也可以预期，与这种类型的其他抗体一样， 新的单克隆抗T3细胞 受体和抗T3抗体将具有多种用途， 其他研究人员在这一领域的人，他们将被给予 自由. 我们计划生产抗鼠V α的单克隆抗体 （特别是V α 11和V α 5）和V β（特别是V β 3和V β 5），针对T细胞受体恒定区 决定簇和T3蛋白。