NON-CONTIGUOUS PATTERNS AND FUNCTIONAL DOMAINS IN DNA

DNA 中的非连续模式和功能域

• 批准号: 3813376
• 负责人: A K KONOPKA
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813376
• 关键词: DNA; computer assisted sequence analysis; genetic mapping; genetic regulatory element; nucleic acid sequence

The vast majority of sequence analysis programs available to date are designed to allow users to detect contiguous sequence fragments that occur more frequently than other contiguous motifs. However, substantial experimental evidence indicates that specific functions of nucleic acids fragments are rarely associated with single, simple sequence motifs. The patterns responsible for biological function are most often non-contiguous and consist of several short oligonucleotides separated by gaps of variable length and composition. In view of the ongoing large sequencing projects reliable procedures for physical mapping of DNA functional domains will be invaluable. To make reliable maps one needs reliable criteria that distinguish between sequences involved in different functions. Non-contiguous pattern analysis already has provided a substantial number of criteria for distinction between protein coding genes, introns, and illegitimate recombination sites. It is believed that by using this methodology various kinds of regulatory regions within repetitive DNA sequences will be mapped unambiguously.

迄今为止，绝大多数可用的序列分析程序都是 旨在允许用户检测出现的连续序列片段 比其他连续的主题更频繁。然而，实质性的 实验证据表明核酸的特定功能 片段很少与单一、简单的序列基序相关。这 负责生物功能的模式通常是不连续的 并由几个由可变间隙分隔的短寡核苷酸组成 长度和组成。鉴于正在进行的大型测序项目 DNA 功能域物理作图的可靠程序将 无价。为了制作可靠的地图，需要可靠的标准 区分涉及不同功能的序列。 非连续模式分析已经提供了大量 区分蛋白质编码基因、内含子和 非法重组位点。据信，通过使用此 重复 DNA 内各种调控区域的方法 序列将被明确地映射。