SOMATIC CELL NON-HOMOLOGOUS RECOMBINATION

体细胞非同源重组

• 批准号: 3813377
• 负责人: A K KONOPKA
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813377
• 关键词: DNA; cell transformation; chemical information system; chemical models; chromosome disorders; computer assisted sequence analysis; computer simulation; gene rearrangement; genetic models; genetic recombination; nucleic acid sequence; restriction fragment length polymorphism

Rare DNA rearrangements that require no extended sequence similarity (of parental molecules) can cause severe genetic alterations of chromosomal fragments in somatic cells. Thus far about 600 parental sequences for this kind of crossing-over are known. It is expected that their number will increase with the rate 50-100 per year. The purpose of this new project is to store available data on illegitimate recombination in one place. Another goal is to provide simple tools for experimental molecular biologists to decide if newly sequenced DNA fragments contain potential recombination 'hot-spots' or not. The research part of this project concerns analysis of known sequences and possible elucidation of mechanisms for non-homologous recombination. Methods of sequence analysis and molecular modeling have been applied thus far.

罕见的DNA重排，不需要扩展的序列相似性（ 亲本分子）可导致染色体的严重遗传改变， 体细胞中的碎片。到目前为止，大约有600个亲本序列 交换类型是已知的。预计他们的人数将 以每年50-100人的速度增长。这个新项目的目的是 在一个地方存储关于非法重组的可用数据。另一 目标是为实验分子生物学家提供简单的工具， 确定新测序的DNA片段是否含有潜在的重组 “热点”或不是。本项目的研究部分涉及分析 已知序列和可能阐明的机制，非同源 重组序列分析和分子建模的方法已经 应用到目前为止。