BIOLOGIC PROPERTIES OF NUCLEAR ONCOGENES AND ATTEMPTS TO BLOCK THEIR EFFECTS

分子生物学的最新发展导致了对 导致激活或失活的特定遗传损伤 各种肿瘤系统中的关键靶基因。这些基因被称为癌基因， 参与细胞生长调控的各个方面。现在是时候了 对了解这些基因发挥作用的确切机制至关重要 因此，最终可以衍生出分子制剂来改变或抑制它们的 效果。 我们选择探索2种蛋白的生物和生化功能 优势(L-myc和c-jun)。的转录和翻译产品 L-myc已被表征，并正在与生物学相关 功能。最终，将对该基因的截短片段进行测试 电位变换抑制功能。 同样，我们最近在文中描述了c-jun的变换函数 哺乳动物细胞，现在正在通过缺失突变来映射这一功能。 这一功能与c-jun的其他已知活动的相关性，例如 将完成激活。能够抑制AP-1的c-jun突变体 将对链的激活和细胞转化进行表征。

Recent developments in molecular biology has led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes in various tumor systems. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth. It is now critical to understand the precise mechanism by which these genes function so molecular agents ultimately can be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant (L-myc and c-jun). Transcriptional and translational products of L-myc have been characterized and are now being correlated with biologic functions. Ultimately, truncated fragments of this gene will be tested for potential transformation suppression function. Likewise, we have recently described the transforming function of c-jun in mammalian cells and are now mapping this function by deletion mutation. Correlation of this function with other known activities of c-jun, such as transactivation will be done. Mutants of c-jun capable of inhibiting AP-1 strans activation and cellular transformation will be characterized.