BIOLOGIC PROTERTIES OF NUCLEAR ONCOGENES

分子生物学在上皮细胞研究中的应用进展 癌症导致了对特定基因损伤的识别 导致关键靶基因的激活或失活。 这些被称为癌基因的基因参与了肿瘤的不同方面。 细胞生长的调节，并因此在早期起主要作用 “启动”和“促进”的致癌过程。现在是时候了 对于了解这些基因的确切机制至关重要 功能，从而最终可以衍生出分子或药物 改变或抑制它们的影响。 我们选择探索2种蛋白的生物和生化功能 显性癌基因(L-myc和c-jun)。转录和翻译 L-MYC的产物可能与生物学特性相关 功能。此外，c-myc和L-myc之间的杂交分子已经 来描述这两个家族的不同之处 会员。最终，对这些基因的结构-功能分析将是 下定决心。 同样，我们最近描述了c-jun的变换函数 在哺乳动物细胞中，现在已经通过缺失映射了这一功能 突变。对转化和反式激活具有重要意义的区域 包括N-末端反式激活结构域、DNA结合区和 亮氨酸拉链领域。此外，c-jun和jun B的比较 将开展激活和改造活动，以 了解这些基因之间的区别。

Recent developments in application of molecular biology to epithelial cancers have led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth and as such play major roles in the early carcinogenic processes of "initiation" and "promotion". It is now critical to understand the precise mechanism by which these genes function so molecular or pharmacologic agents can ultimately be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant oncogenes (L-myc and c-jun). Transcriptional and translational products of L-myc may been characterized and correlated with biologic functions. In addition, hybrid molecules between c-myc and L-myc have been made to characterize the differences between these two family members. Ultimately, structure-function analysis for these genes will be determined. Likewise, we have recently described the transforming function of c-jun in mammalian cells and have now mapped this function by deletion mutation. Regions important for transformation and transactivation include an N-terminal transactivation domain, DNA binding region, and the leucine zipper domain. In addition, comparisons of c-jun with Jun B transactivation and transformation activities will be undertaken to understand the difference between these genes.