BIOLOGIC PROPERTIES OF NUCLEAR ONCOGENES AND ATTEMPTS TO BLOCK THEIR EFFECTS

分子生物学的最新发展导致了对 特定的遗传损伤会导致激活或 不同肿瘤系统中关键靶基因的失活。这些基因， 被称为癌基因，参与多种方面的调控。 细胞生长。现在关键是要通过以下方式来理解精确的机制 这些基因的功能，所以最终可以派生出分子制剂 改变或抑制它们的影响。 我们选择探索2种蛋白的生物和生化功能 优势(L-myc和c-jun)。转录和翻译产品 L-myc的特征，现在正在与 生物功能。最终，这种基因的截短片段将 测试潜在的相变抑制功能。 同样，我们最近描述了c-jun的变换函数 在哺乳动物细胞中，现在通过删除来映射这一功能 突变。这一功能与其他已知活动的相关性 C-JUN等都会被激活。具有c-jun功能的突变体 抑制AP-1的反式激活和细胞转化将是 特色化的。

Recent developments in molecular biology has led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes in various tumor systems. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth. It is now critical to understand the precise mechanism by which these genes function so molecular agents ultimately can be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant (L-myc and c-jun) . Transcriptional and translational products of L-myc have been characterized and are now being correlated with biologic functions. Ultimately, truncated fragments of this gene will be tested for potential transformation suppression function. Likewise, we have recently described the transforming function of c-jun in mammalian cells and are now mapping this function by deletion mutation. Correlation of this function with other known activities of c-jun, such as transactivation will be done. Mutants of c-jun capable of inhibiting AP-1 transactivation and cellular transformation will be characterized.