BIOLOGIC PROPERTIES OF NUCLEAR ONCOGENES AND ATTEMPTS TO BLOCK THEIR EFFECTS

分子生物学的最新发展导致了 特定的遗传损伤导致激活或 各种肿瘤系统中关键靶基因的失活。 这些基因， 称为癌基因，参与调控的各个方面， 细胞生长 现在关键是要了解确切的机制， 这些基因的功能使得最终可以衍生出 来改变或抑制它们的作用。 我们选择探索2的生物学和生化功能， 显性（L-myc和c-jun）。转录和翻译产物 的L-myc已经被表征，现在正在与 生物功能。 最终，该基因的截短片段将 测试电位转换抑制功能。 同样，我们最近描述了c-jun的转化功能 现在正在通过删除来定位这种功能 突变 该功能与其他已知活动的相关性 c-jun，如反式激活将被完成。 c-jun突变体 抑制AP-1反式激活和细胞转化的作用将是 表征了

Recent developments in molecular biology has led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes in various tumor systems. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth. It is now critical to understand the precise mechanism by which these genes function so molecular agents ultimately can be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant (L-myc and c-jun) . Transcriptional and translational products of L-myc have been characterized and are now being correlated with biologic functions. Ultimately, truncated fragments of this gene will be tested for potential transformation suppression function. Likewise, we have recently described the transforming function of c-jun in mammalian cells and are now mapping this function by deletion mutation. Correlation of this function with other known activities of c-jun, such as transactivation will be done. Mutants of c-jun capable of inhibiting AP-1 transactivation and cellular transformation will be characterized.