REGULATION OF TRANSCRIPTION IN SV40 AND ADENOVIRUS

DNA肿瘤病毒的转化区。SV40和JCV，以及 腺病毒调节病毒和细胞基因的转录。这个 这些研究的目的是探讨T/T改变的机制。 抗原(S)和E1a蛋白调控转录及其与蛋白质的关系 细胞转化。在腺病毒中，EIIA上游序列 含有蛋白质ATF和EIIF的结合部位，作为增强剂和 可被EIA和SV40T/t抗原反式激活。特定的 ATF和EIIF结合位点的突变抑制EIIA 增强器200倍。插入突变的分析表明，空间 上游ATF和EIIF结合位点与 DNA螺旋上的EIIF下游结合部位很重要。与一致 之前的发现，使用凝胶位移分析，我们证明了结合 野生型腺病毒感染后EIIF活性增加。在……里面 相反，在相同的凝胶位移条件下，ATF的结合活性 会因病毒感染而减少。因为ATF实际上是一个亲密的家庭 相关转录因子的研究正在进行中，以期 对腺病毒感染的不同调控。

The transforming region of the DNA tumor viruses. SV40 and JCV, and adenovirus regulates transcription of viral and cellular genes. The purpose of these studies is to investigate the mechanism by which the T/t antigen(s) and E1A proteins modulate transcription and its relationship to cellular transformation. In adenovirus, The EIIA upstream sequences which contain the binding sites for proteins ATF and EIIF act as an enhancer and can be trans-activated by both EIA and SV40 T/t antigens. Specific mutation of either the ATF and EIIF binding sites inhibited the EIIA enhancer 200-fold. Analysis of insertion mutation suggests that the spatial alignment of the upstream ATF and EIIF binding sites with respect to the downstream EIIF binding site on the DNA helix is important. Consistent with previous findings, using gel shift analysis we demonstrate that the binding activity of EIIF is increased following wild-type adenovirus infection. In contrast, using identical gel shift conditions, the binding activity of ATF is decreased by viral infection . Since ATF is actually a family of closely related transcription factors, studies are in progress to look at differential regulation in response to adenovirus infection.