MOLECULAR STRUCTURE OF HAV--GENOME SEQUENCE AND ORGANIZATION

甲型肝炎病毒的分子结构--基因组序列和组织

• 批准号: 3818222
• 负责人: J I COHEN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818222
• 关键词: Hepatovirus; attenuated microorganism; complementary DNA; gene deletion mutation; genetic mapping; genetic translation; genome; molecular cloning; nucleic acid sequence; protein sequence; tissue /cell culture; virus RNA; virus envelope; virus genetics; virus protein

RNAs transcribed from the cDNA clones of wild-type, cell culture adapted hepatitis A virus, or mutants constructed from these clones are being translated in vitro to define the parameters affecting the efficiency of translation. Deletion mutants have been constructed to answer the question of whether hepatitis A viability requires an intact VP4 gene or whether myristylation of VP4 is important.

从野生型细胞培养物的cDNA克隆转录的RNA 适应的甲型肝炎病毒，或从这些克隆构建的突变体 在体外被翻译来定义影响 翻译的效率。 缺失突变体已经被 构建来回答甲型肝炎是否存活的问题 需要完整的VP4基因，或者VP4的肉豆蔻酰化是否 重要.