课题基金 / 基金详情

CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION

CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION
用于转染的全长甲型肝炎病毒 CDNA 的构建
批准号：
4688549
负责人：
J I COHEN
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4688549
关键词：
Hepatovirus complementary DNA genetic manipulation genetic mapping genome molecular cloning plasmids simian virus 40 tissue /cell culture virus genetics

项目摘要

项目成果

J I COHEN的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
cDNA from hepatitis A virus (HAV) has been cloned into pBR 322. Six cDNA clones which together span the entire genome of HAV have been isolated. These clones have been ligated together to form a single clone which was thought to represent a cDNA analog of full-length HAV in pBR 322. In addition, the HAV cDNA was inserted into an SV40 vector. Transfection of both tissue culture cells (in vitro) and marmosets (in vivo) with these plasmids failed to generate HAV. Fine structure mapping of the HAV cDNA subsequently indicated that about 40 base pairs had been deleted during the ligation process. Construction of a full-length infectious HAV cDNA clone is in progress.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
MOLECULAR GENETICS OF VARICELLA ZOSTER VIRUS
CELLULAR HOMOLOGS OF HERPESVIRUS GENES
MOLECULAR BIOLOGY OF VARICELLA ZOSTER VIRUS INFECTIONS
MOLECULAR BIOLOGY OF TRANSFORMATION BY EPSTEIN-BARR VIRUS
海外基金