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CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION

CONSTRUCTION OF FULL LENGTH HEPATITIS A VIRUS CDNA FOR TRANSFECTION
用于转染的全长甲型肝炎病毒 CDNA 的构建
批准号：
4688549
负责人：
J I COHEN
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4688549
关键词：
Hepatovirus complementary DNA genetic manipulation genetic mapping genome molecular cloning plasmids simian virus 40 tissue /cell culture virus genetics

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中文摘要
翻译
将甲型肝炎病毒（HAV）的cDNA克隆到pBR 322中。 六个cDNA 已经分离出一起跨越HAV整个基因组的克隆。 将这些克隆连接在一起形成单个克隆， 被认为代表pBR 322中全长HAV的cDNA类似物。 在 另外，将HAV cDNA插入到SV40载体中。 转染 组织培养细胞（体外）和绒猴（体内）都具有这些 质粒不能产生HAV。 甲型肝炎病毒cDNA的精细结构定位 随后表明，大约40个碱基对已被删除， 连接过程。 甲型肝炎病毒感染性全长cDNA克隆的构建 正在进行
英文摘要
cDNA from hepatitis A virus (HAV) has been cloned into pBR 322. Six cDNA clones which together span the entire genome of HAV have been isolated. These clones have been ligated together to form a single clone which was thought to represent a cDNA analog of full-length HAV in pBR 322. In addition, the HAV cDNA was inserted into an SV40 vector. Transfection of both tissue culture cells (in vitro) and marmosets (in vivo) with these plasmids failed to generate HAV. Fine structure mapping of the HAV cDNA subsequently indicated that about 40 base pairs had been deleted during the ligation process. Construction of a full-length infectious HAV cDNA clone is in progress.
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