REPLICATION OF HEPATITIS A VIRUS IN VIVO

甲型肝炎病毒的体内复制

• 批准号: 3822094
• 负责人: J I COHEN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822094
• 关键词: Hepatovirus; biopsy; blood chemistry; cell population study; cellular immunity; immunofluorescence technique; immunogenetics; lymphocyte; tissue /cell culture; virus replication

Extra-hepatic sites of hepatitis A virus replication have been examined during experimental infection. A chimpanzee was inoculated orally with wild-type HAV HM-175 and seroconverted to HAV 23 days after inoculation. Serum, saliva, and serum throat swabs were inoculated into seronegative marmosets. HAV was present in the 18 days after inoculation, in saliva 23 days after inoculation, and in throat swabs 18 to 21 days after inoculation. Additional specimens will be tested using cDNA hybridization and fluorescein-labeled antibody.

甲型肝炎病毒复制的肝外部位已被 在实验感染期间检查。 一只黑猩猩 经口接种野生型HAV HM-175并血清转化 接种后23天， 血清、唾液和血清 将咽拭子接种到血清阴性的绒猴中。 HAV 在接种后18天出现，23天出现在唾液中 接种后18至21天， 接种。 将使用cDNA检测其他标本 杂交和荧光素标记抗体。