STRUCTURE-FUNCTION RELATIONSHIP OF BETA 1-4 GALACTOSYLTRANSFERASE

Beta 1-4 半乳糖基转移酶的结构-功能关系

• 批准号: 3916338
• 负责人: P K QASBA
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916338
• 关键词: complementary DNA; enzyme mechanism; galactosyltransferases; genetic manipulation; genetic promoter element; nucleic acid sequence; protein engineering; protein structure function

Studies on the structure-function relationship of proteins, specifically of galactosyltransferases and their modifiers like alpha-lactalbumin, have been initiated. For such studies, we are producing the normal and altered proteins by the recombinant DNA methodology. A T7-promoter and a polylinker DNA sequence was inserted into a baculovirus vector adjacent to the polyhydrin promoter. Such constructs are used for the production of normal and altered proteins in, a) baculovirus infected insect cells, or b) frog oocytes by injecting the cDNA specific mRNA transcribed in vitro by T7-polymerase. We have also engineered the cDNA sequences of galactosyltransferase and alpha-lactalbumin, in such a way that the expression of the cDNA sequence have a potential to produce either soluble and secreted forms, or membrane anchored forms of the protein.

蛋白质结构-功能关系的研究， 特别是半乳糖基转移酶及其修饰物，如 α-乳清蛋白，已经启动。对于这样的研究，我们是 利用重组DNA生产正常蛋白和变异蛋白 方法论。T7启动子和多联体DNA序列 插入到与聚水化合物相邻的杆状病毒载体中 推动者。这样的构造被用于生产正常的 和改变的蛋白质，a)杆状病毒感染的昆虫细胞，或 B)通过注射转录于 T7-聚合酶体外扩增。我们还对cdna序列进行了改造。 半乳糖基转移酶和α-乳清蛋白，以这种方式 该c DNA序列的表达具有生产潜力 可溶型和分泌型，或膜锚定型 蛋白质。