STRUCTURE-FUNCTION RELATIONSHIP OF BETA 1-4 GALACTOSYLTRANSFERASE

Beta 1-4 半乳糖基转移酶的结构-功能关系

• 批准号: 3916338
• 负责人: P K QASBA
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916338
• 关键词: complementary DNA; enzyme mechanism; galactosyltransferases; genetic manipulation; genetic promoter element; nucleic acid sequence; protein engineering; protein structure function

Studies on the structure-function relationship of proteins, specifically of galactosyltransferases and their modifiers like alpha-lactalbumin, have been initiated. For such studies, we are producing the normal and altered proteins by the recombinant DNA methodology. A T7-promoter and a polylinker DNA sequence was inserted into a baculovirus vector adjacent to the polyhydrin promoter. Such constructs are used for the production of normal and altered proteins in, a) baculovirus infected insect cells, or b) frog oocytes by injecting the cDNA specific mRNA transcribed in vitro by T7-polymerase. We have also engineered the cDNA sequences of galactosyltransferase and alpha-lactalbumin, in such a way that the expression of the cDNA sequence have a potential to produce either soluble and secreted forms, or membrane anchored forms of the protein.

蛋白质结构与功能关系的研究， 特别是半乳糖基转移酶及其修饰物， α-乳白蛋白已经启动。 对于这些研究，我们 通过重组DNA产生正常和改变的蛋白质 方法论 T7-启动子和多接头DNA序列是 插入到杆状病毒载体中， 启动子 这样的构建体用于生产正常的 a）杆状病毒感染的昆虫细胞，或 B）通过注射在卵母细胞中转录的cDNA特异性mRNA， 体外T7聚合酶。 我们还设计了cDNA序列 半乳糖基转移酶和α-乳白蛋白， cDNA序列的表达具有产生 或者是可溶的和分泌的形式，或者是膜锚定的形式， 蛋白质。