EXPRESSION OF BETA 1-4 GALTRANSFERASE

几种病毒的激素需求和组织特异性表达 β-1-4半乳糖基转移酶的分子形式正在研究中。 在乳腺中，它的表达受催乳激素的调节 并且还依赖于 乳房组织。 该项目的主要目标是确定具有以下特点的细胞系： A)不产生1-4半转移酶，b)产生非常低的水平， 和c)非常高的酶水平。这样的细胞系将是 用于转基因实验以确定其结构- 半乳糖转移酶和白蛋白的功能关系，以及 还有荷尔蒙对该酶表达的调节。 在目前的研究中，我们正在：a)筛选几个细胞系 1)1-4半转移酶的活性；2)1-4半转移酶的分子形式 蛋白质的产生，3)酶的mRNA水平，以及4)mRNA的大小 B)研究类固醇和类固醇的作用 有丝分裂激素及其组合对任何参数的影响 A)中所述；C)确定细胞周期依赖的表达 瑞士小鼠3T3细胞中的酶。

Hormonal requirement and tissue specific expression of several molecular forms of beta-1-4 galtransferase is being investigated. In mammary gland its expression is modulated by lactogenic hormones and is also dependent on the functional differentiation of the mammary tissue. The primary goal of this project is to identify cell lines which: a) do not produce 1-4 galtransferase, b) produce very low levels, and c) very high levels of the enzyme. Such cell lines will be used for transfection experiments to determine the structure- function relationship of galtransferase and alphalactalbumin, and also the hormonal regulation of expression of the enzyme. In the present study we are: A) screening several cell lines for 1) the activity of 1-4 galtransferase, 2) the molecular form of the protein produced, 3) mRNA levels for the enzyme, and 4) mRNA size class produced; B) investigating the effect of steroidal and mitogenic hormones and their combinations on any of the parameters stated in A); C) determining cell-cycle dependent expression of the enzyme in Swiss mouse 3T3 cells.