EXPRESSION OF BETA 1-4 GALTRANSFERASE

激素需求和组织特异性表达的几个 正在研究β-1-4半乳糖转移酶的分子形式。 在乳腺中，其表达受催乳激素调节 并且还取决于细胞的功能分化， 乳腺组织 本项目的主要目标是鉴定以下细胞系： a）不产生1-4半乳糖转移酶，B）产生非常低的水平， 和c）非常高水平的酶。 这样的细胞系将是 用于转染实验以确定结构- 半乳糖转移酶与乳清蛋白的功能关系， 也是酶表达的激素调节。 在本研究中，我们：A）筛选几种细胞系， 1)1-4半乳糖转移酶的活性，2）1 - 4半乳糖转移酶的分子形式， 产生的蛋白质，3）酶的mRNA水平，和4）mRNA大小 B）研究甾体类和 促有丝分裂激素及其组合对任何参数的影响 C）确定所述细胞的细胞周期依赖性表达， 瑞士小鼠3 T3细胞中的酶。

Hormonal requirement and tissue specific expression of several molecular forms of beta-1-4 galtransferase is being investigated. In mammary gland its expression is modulated by lactogenic hormones and is also dependent on the functional differentiation of the mammary tissue. The primary goal of this project is to identify cell lines which: a) do not produce 1-4 galtransferase, b) produce very low levels, and c) very high levels of the enzyme. Such cell lines will be used for transfection experiments to determine the structure- function relationship of galtransferase and alphalactalbumin, and also the hormonal regulation of expression of the enzyme. In the present study we are: A) screening several cell lines for 1) the activity of 1-4 galtransferase, 2) the molecular form of the protein produced, 3) mRNA levels for the enzyme, and 4) mRNA size class produced; B) investigating the effect of steroidal and mitogenic hormones and their combinations on any of the parameters stated in A); C) determining cell-cycle dependent expression of the enzyme in Swiss mouse 3T3 cells.