ROLE OF COLLAGENOLYTIC METALLOPROTEINASES IN METASTASES

为了研究IV型的分子特性， 胶原金属蛋白酶，我们获得了一级序列 信息和使用这些数据，开发了单特异性 抗体的 序列信息识别IV型胶原蛋白 金属蛋白酶是一种独特的酶，与其他酶具有一定的同源性， 细胞外基质降解金属蛋白酶。 合成 使用该序列信息产生的肽用于 制造抗IV型胶原蛋白溶解金属蛋白酶抗体。 这些抗体用于研究 激活胶原酶并筛选表达文库 以获得cDNA克隆。

In order to investigate the molecular properties of the type IV collagen metalloproteinase, we have obtained primary sequence information and using this data, have developed monospecific antibodies. The sequence inforaation identifies type IV collagen metalloproteinase as a unique enzyme with some homology to other extracellular matrix degrading metalloproteinases. Synthetic peptides generated using this sequence information were used to make anti-type IV collagenolytic metalloproteinase antibodies. These antibodies were used to investigate the mechanism of activation of the collagenase and to screen an expression library to obtain a cDNA clone.