IMMUNOBIOLOGY OF ALEUTIAN DISEASE

单克隆抗体用于研究抗原差异 并在体外表征病毒蛋白 和体内。 高毒力犹他州I ADV被清楚地描绘出来 来自组织培养适应的无毒ADV-G菌株。 这 特异性可通过间接免疫荧光证实 (IFA)针对克兰德尔猫肾细胞的感染培养物， 针对犹他州I型ADV感染貂的组织。 利用鉴定的各种mAb进行免疫沉淀分析 特异性抗原决定簇。 当免疫沉淀- 定义的反应性与IFA组织和体外 反应模式很明显，病毒相关的 体内识别的抗原决定簇是蛋白水解的 病毒结构蛋白的产物。 因此，完整的结构ADV 在体内没有鉴定出蛋白质。 然而，结构蛋白 当ADV-G或犹他州I ADV感染时， 分析CRFK细胞。

Monoclonal antibodies were used to study antigenic differences among strains of ADV and to characterize viral proteins in vitro and in vivo. Highly virulent Utah I ADV was clearly delineated from the tissue culture-adapted avirulent ADV-G strain. This specificity could be demonstrated by indirect immunofluorescence (IFA) against infected cultures of Crandell feline kidney cells or against tissues of Utah I ADV-infected mink. Immunoprecipitation analyses utilizing various mAbs identified specific antigenic determinants. When immunoprecipitation- defined reactivities were correlated with IFA tissue and in vitro patterns of reactivity it was apparent that the virus-associated antigenic determinants recognized in vivo were proteolytic products of viral structural proteins. Thus, intact structural ADV proteins were not identified in vivo. However, structural proteins were detected in vitro when ADV-G or Utah I ADV-infected CRFK cells were analyzed.