IMMUNOBIOLOGY OF ALEUTIAN DISEASE

用单抗研究了两种病毒的抗原性差异。 并对体外和体内的病毒蛋白进行鉴定。 从组织中清楚地描绘出强毒的犹他州I型ADV 培养适应的ADV-G强毒株。这种专一性可能是 通过间接免疫荧光(IFA)检测受感染的培养物 克兰德尔猫肾细胞或抗犹他州I型腺病毒感染的组织 貂皮。利用已鉴定的多种单抗进行免疫沉淀分析 特定的抗原决定簇。当免疫沉淀定义为 反应性与IFA组织和体外模式相关 反应性很明显，病毒相关的抗原决定簇 体内识别的是病毒结构蛋白的蛋白水解物。 因此，在体内没有鉴定出完整的结构蛋白。然而， ADV-G或犹他州I期体外检测到结构蛋白 对感染ADV的CRFK细胞进行分析。

Monoclonal antibodies were used to study antigenic differences among strains of ADV and to characterize viral proteins in vitro and in vivo. Highly virulent Utah I ADV was clearly delineated from the tissue culture-adapted avirulent ADV-G strain. This specificity could be demonstrated by indirect immunofluorescence (IFA) against infected cultures of Crandell feline kidney cells or against tissues of Utah I ADV-infected mink. Immunoprecipitation analyses utilizing various mAbs identified specific antigenic determinants. When immunoprecipitation-defined reactivities were correlated with IFA tissue and in vitro patterns of reactivity it was apparent that the virus-associated antigenic determinants recognized in vivo were proteolytic products of viral structural proteins. Thus, intact structural ADV proteins were not identified in vivo. However, structural proteins were detected in vitro when ADV-G or Utah I ADV-infected CRFK cells were analyzed.