IMMUNOBIOLOGY OF ALEUTIAN DISEASE

这个项目的目标是定义免疫机制和病毒 在阿留申病(AD)发病机制中的重要特征。 用单抗研究了两种病毒的抗原性差异。 并对体外和体内的病毒蛋白进行鉴定。 从组织中清楚地描绘出强毒的犹他州I型ADV 培养适应的ADV-G强毒株。这种专一性可能是 通过间接免疫荧光(IFA)证明对感染的 克兰德尔猫肾细胞的培养或与犹他州的组织对抗 感染ADV的水貂。利用多种单抗进行免疫沉淀分析 确定了特定的抗原决定簇。什么时候 免疫沉淀确定的反应性与IFA组织相关 在体外的反应模式中，很明显 体内识别的病毒相关抗原决定簇是蛋白水解性的 病毒结构蛋白的产物。完整的结构ADV蛋白 在活体内未鉴定。然而，在体内检测到结构蛋白 体外分析ADV-G和Utah I ADV感染CRFK细胞的时间。因此， 在体内发生蛋白分解，产生小的ADV相关多肽 但在体外并没有重大的发现，ADV结构蛋白 是主要的病毒抗原。

The goal of this project is to define immune mechanisms and viral characteristics important in the pathogenesis of Aleutian disease (AD). Monoclonal antibodies were used to study antigenic differences among strains of ADV and to characterize viral proteins in vitro and in vivo. Highly virulent Utah I ADV was clearly delineated from the tissue culture-adapted avirulent ADV-G strain. This specificity could be demonstrated by indirect immunofluorescence (IFA) against infected cultures of Crandell feline kidney cells or against tissues of Utah I ADV-infected mink. Immunoprecipitation analyses utilizing various mAbs identified specific antigenic determinants. When immunoprecipitation-defined reactivities were correlated with IFA tissue and in vitro patterns of reactivity it was apparent that the virus-associated antigenic determinants recognized in vivo were proteolytic products of viral structural proteins. Intact structural ADV proteins were not identified in vivo. However, structural proteins were detected in vitro when ADV-G or Utah I ADV-infected CRFK cells were analyzed. Thus, proteolysis occurred in vivo, resulting in small ADV related polypeptides but was not a significant finding in vitro where ADV structural proteins were the predominant viral antigen.