MODULATION OF MYELOID TUMOR CELL GROWTH IN VITRO BY ESTROGEN/PROGESTERONE ANALOGS

骨髓性白血病已被证明是特别没有反应， 糖皮质激素治疗，一种在治疗中非常有用的方案， 治疗T和B细胞白血病。我们已经研究了体外 几种骨髓肿瘤细胞系，包括HL 60和U937， 各种性类固醇，包括雌二醇，己烯雌酚（DES）， 孕酮和睾酮。 所有测试的细胞系都被证明被杀死 雌二醇、己烯雌酚和孕酮的含量范围为10- 100 μ M。 DES 被证明是最有效的。细胞死亡的方法似乎包括 细胞质肿胀和细胞核挤出，伴有DNA 降解 对氢化可的松和地塞米松敏感的白血病， 如T和B细胞白血病，也对DES非常敏感， 雌二醇和孕酮。 发现所有检测的细胞系均为 雌二醇受体（DES共有），但没有雌二醇受体。 可检测到孕酮。药物有效性体内研究 针对无胸腺小鼠中骨髓肿瘤异种移植物生长的研究正在进行中。

Myeloid leukemias have proven to be particularly unresponsive to glucocorticoid therapy, a regimen shown to be quite useful in the treatment of T and B cell leukemias.We have investigated the in vitro sensitivity of several myeloid tumor cell lines, including HL60 and U937, to various sex steroids, including estradiol, diethylstilbestrol (DES), progesterone and testosterone. All cell lines tested proved to be killed by amounts of estradiol, DES and progesterone ranging from 10-100uM. DES proved the most effective. The method of cell death appears to involve cytoplasmic swelling and extrusion of nuclei, accompanied by DNA degradation. Leukemias sensitive to hydrocortisone and dexamethasone, such as T and B cell leukemias, were also quite sensitive to DES, estradiol and progesterone. All cell lines tested were found to posses receptors for estradiol (which are shared by DES), but no receptors for progesterone could be detected. in vivo studies of drug effectiveness against myeloid tumor xenograph growth in athymic mice are underway.