REGULATION OF CELL GROWTH BY TRANSFERRIN RECEPTORS

转铁蛋白受体对细胞生长的调节

• 批准号: 3962905
• 负责人: L M NECKERS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3962905
• 关键词: cell growth regulation; cyclic AMP; growth inhibitors; immunohematology; leukocyte activation /transformation; lymphoblast; lymphocyte; messenger RNA; oncogenes; tissue /cell culture; transferrin

All cells studied to date require transferrin for growth. We and others have shown that antibodies to the transferrin receptor block the growth of lymphoblastoid cell lines. In mitogen-stimulated lymphocytes, these antibodies block proliferation. We are studying the processes which regulate the appearance of these receptors in lymphocytes and lymphoblastoid cell lines, and the function of these receptors in cell growth and metabolism. We have demonstrated that G1 arrest occurs in both normal and malignant cells when transferrin receptors are blocked, even if cells are expressing high levels of growth factor receptor messenger RNA and c-myc and c-myb messenger RNA. Furthermore, either blockade of calcium channels or addition of cAMP to cells results in G1 arrest and loss of transferrin receptor mRNA. The effect of cAMP can be detected at the level of transcription.

到目前为止，所有研究的细胞都需要转铁蛋白来生长。我们和其他人 已经表明，转铁蛋白受体的抗体可以阻止血管生长 淋巴母细胞系。在有丝分裂原刺激的淋巴细胞中，这些 抗体可以阻止细胞增殖。我们正在研究的过程是 调节淋巴细胞中这些受体的出现，并 淋巴母细胞系及其受体在细胞中的功能 生长和新陈代谢。我们已经证明在两种情况下都会发生G1期停滞 当转铁蛋白受体被阻断时，正常和恶性细胞，即使 细胞表达高水平的生长因子受体信使RNA C-myc和c-myb信使RNA。此外，无论是钙离子的阻断 通道或将cAMP添加到细胞中会导致G1期停滞和 转铁蛋白受体基因。CAMP的作用可以在以下水平上检测到 抄写。