MOLECULAR IMMUNOLOGY

在过去的一年里，在体内模拟方面取得了一些进展 利用分子生物学方法产生抗体。最多的 重大突破是噬菌体Fab/Fv展示的发展 图书馆技术。有兴趣探索的应用 这项技术有助于癌症研究和抗体工程。一种抗ETS2病毒 单抗T-7轻链，U-244重链，推测 已克隆了T-7和U-244的重链相关序列 成功了。一种新的FAB组合库构建策略 已经设计了一个更多样化的曲目。小鼠脾抗ERG抗体 CDNA将通过这一策略进行克隆和筛选。噬菌体Fab 已向MRC请求显示向量。一种防ERG的FAB显示器 将制作库来验证系统的效率。一些BALB/c 用几种临床肿瘤样本和它们的抗体免疫小鼠 正常对照组。将制作FAB展示文库，并将那些抗肿瘤 标记克隆将通过平移或亲和层析进行筛选。为 高亲和力Fab，一种产生随机突变的新方法 所需的域已经开始，这项工作将继续。一种方法 通过以下方式产生新一代治疗性单抗 结合与抗体可变区相关的所需特异性 所定义的抗体效应器功能与某些 设计了单抗恒定域。几个 将对单抗细胞系进行鉴定。

In the past year, some advances have been made in mimicking in vivo antibody generation by use of molecular biology approaches. The most significant breakthrough was the development of the phage Fab/Fv display library technology. There is interest in exploring the application of this technology to cancer research and antibody engineering. An anti-ETS2 monoclonal antibody T-7 light chain, U-244 heavy chain, and a presumably heavy chain-related sequence from both T-7 and U-244, have been cloned successfully. A new Fab combinatorial library construction strategy for a more diverse repertoire has been designed. Mouse splenic anti-erg Fab cDNAs are to be cloned and screened by this strategy. The phage Fab display vector has been requested from MRC. An anti-erg Fab display library will be made to verify the efficiency of the system. Some BALB/c mice have been immunized with several clinical tumor samples and their normal controls. Fab display libraries will be made, and those anti-tumor marker clones will be selected by panning or affinity chromatography. For high-affinity Fab, a new approach to generate random mutagenesis to desired domains has been started and this work will continue. An approach to generate a new generation of therapeutic monoclonal antibodies by combining desired specificity associated with antibody variable domains with the defined antibody effector function associated with some monoclonal antibody constant domains has been designed. Several monoclonal antibody cell lines are to be characterized.