CELL ADHESION MOLECULES IN OCULAR INFLAMMATION

细胞粘附分子是介导结合的表面蛋白 细胞与细胞外基质和其他细胞的相互作用。 的表达 细胞粘附分子，这是重要的迁移， 白细胞到炎症部位，部分受 细胞因子的分泌。 我们正在研究细胞的表达 粘附分子在眼部炎症中的作用， 阻断细胞粘附分子作为葡萄膜炎的治疗， 其他眼部炎症性疾病。 在过去的一年里，我们已经证明， 抗Mac-1抗体抑制内毒素诱导的 小鼠的葡萄膜炎。 我们后来证明单克隆抗体 阻断细胞间粘附分子-1（ICAM-1）和淋巴细胞 功能相关抗原-1（LFA-1）可抑制 内毒素诱导的大鼠葡萄膜炎和实验性自身免疫性葡萄膜炎 对小鼠 这些抗ICAM-1和LFA-1的抗体也抑制了ICAM-1和LFA-1的表达。 体外淋巴细胞刺激。 最后，研究细胞表达 葡萄膜炎动物模型中的粘附分子显示， ICAM-1在炎性细胞浸润前表达于眼内， 细胞 在对人类活检组织的研究中，我们证明了 ICAM-1、E-选择素和血管细胞粘附分子-1（VCAM-1） 角膜移植失败 ICAM-1表达最强的是 在患有最严重炎症疾病的角膜样本中， 表明细胞粘附分子表达在 角膜移植失败 我们之前已经证明了 后葡萄膜炎患者细胞粘附分子的变化， E-选择素在动物眼前段的表达 内毒素诱发的葡萄膜炎

Cell adhesion molecules are surface proteins that mediate the binding of cells to extracellular matrix and to other cells. The expression of cell adhesion molecules, which is important for the migration of leukocytes to sites of inflammation, is partly regulated by the secretion of cytokines. We are studying the expression of cell adhesion molecules in ocular inflammation and investigating the blocking of cell adhesion molecules as a treatment for uveitis and other ocular inflammatory diseases. Over the past year we have shown that treatment with a monoclonal antibody against Mac-1 inhibits the development of endotoxin-induced uveitis in mice. We later demonstrated that monoclonal antibodies blocking intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) inhibits the development of both endotoxin-induced uveitis in rats and experimental autoimmune uveitis in mice. These antibodies against ICAM-1 and LFA-1 also inhibited lymphocyte stimulation in vitro. Finally, studies of expression of cell adhesion molecules in animal models of uveitis have shown that ICAM-1 is expressed in the eye before the infiltration of inflammatory cells. In studies of human biopsy tissue, we demonstrated the expression of ICAM-1, E-selectin, and vascular cell adhesion molecule-1 (VCAM-1) in corneal graft failure. The strongest expression of ICAM-1 occurred in corneal specimens with the greatest inflammatory disease, suggesting that cell adhesion molecule expression plays a role in corneal graft failure. We previously had demonstrated the expression of cell adhesion molecules in human eyes with posterior uveitis and the expression of E-selectin in the anterior segment of animal eyes with endotoxin-induced uveitis.