ONTOGENY OF THE LUTINIZING HORMONE RELEASING HORMONE SYSTEM

黄化激素释放激素系统的个体发育

• 批准号: 3846300
• 负责人: S WRAY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3846300
• 关键词: cell migration; central nervous system; developmental neurobiology; ectoderm; embryo /fetus tissue /cell culture; fibronectins; gene expression; genetically modified animals; laboratory mouse; neural cell adhesion molecules; neurons; posttranslational modifications

LHRH neurons are derived from the olfactory placode and migrate into the brain. The factors which dictate cell-specific LHRH gene expression and the migratory mechanism(s) used by the LHRH neurons during their movement in the CNS are currently unknown. Using in vivo and in vitro models, we are examining the prenatal development of the LHRH system. We are testing the hypothesis that N-CAM and fibronectin direct LHRH movement in nasal regions by using embryonic explants which maintain large numbers of LHRH expressing cells. In addition, we have found that LHRH cells do not posttranslationally modify LHRH to the amidated form in vivo until reaching the forebrain. Embryonic LHRH cells derived from nasal regions were able to produce the "mature" peptide in vitro in the absence of brain tissue, suggesting that this is a time-dependent (but not spatially-dependent) event.

LHRH神经元来源于嗅基板，并迁移到 个脑袋 决定细胞特异性LHRH基因表达的因素以及 LHRH神经元在运动过程中使用的迁移机制 目前尚不清楚。 使用体内和体外模型，我们 正在研究LHRH系统的产前发育。 我们 检验N-CAM和纤连蛋白指导LHRH运动的假设， 鼻区通过使用胚胎外植体，保持大量的 LHRH表达细胞。 此外，我们发现LHRH细胞不 在体内将LHRH后修饰为酰胺化形式， 到达前脑 鼻区来源的胚胎LHRH细胞 能够在体外产生“成熟”肽， 脑组织，这表明这是一个时间依赖性（但不是 空间相关）事件。