IMMUNOLOGICAL ASPECTS OF NERVOUS SYSTEM INFECTION WITH HIV

HIV 神经系统感染的免疫学方面

• 批准号: 3846955
• 负责人: D E GRIFFIN
• 金额: --
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3846955
• 关键词: HIV infections; antiviral antibody; blood; cerebrospinal fluid; cytokine; flow cytometry; histochemistry /cytochemistry; human immunodeficiency virus; inflammation; laboratory mouse; leukocyte activation /transformation; macrophage; monocyte; nervous system disorder; neuroimmunomodulation; opportunistic infections; virus antigen; virus genetics; virus replication

It is hypothesized that different HIV-associated neurological diseases have different pathogeneses. In some diseases the immune system is expected to play a primary role, in others a secondary role, and in others no role. To identify the participation of the immune response in various HIV-associated neurological diseases we propose (1) To identify the inflammatory cells and the state of activation of these cells by studying mononuclear cells in the cerebrospinal fluid, blood and tissues using flow cytometry and immunoperoxidase staining. (2) To determine the local production of inflammatory mediators in different sites using immunological and biological assays to detect and quantitate these cell products and nucleic acid hybridization assays to quantitate the corresponding mRNA. (3) To determine the effect of HIV infection on the function of human monocyte/macrophages and their production of inflammatory mediators and the portion of the long terminal repeat controlling HIV transcription in monocytes by infecting monocytes and monocyte cell lines with HIV and HIV constructs. (4) To determine whether activated lymphocytes traffic nonspecifically to the CNS independent of the presence of antigen in the CNS using passive transfer into mice of different activated cell populations purified by sedimentation and fluorescence activated cell sorting. (5) To determine the presence and level of the HIV-specific and neural tissue-specific immune responses by assaying for the presence of specific antibody in serum and CSF.

据推测，不同的HIV相关神经学 疾病有不同的病机。在某些疾病中，免疫 预计系统将发挥主要作用，其他系统将发挥次要作用 角色，而在其他人中没有角色。以确定参与 多种HIV相关神经系统疾病的免疫反应 建议(1)鉴定炎性细胞及其状态 通过研究单个核细胞对这些细胞的激活作用 脑脊液、血液和组织的流式细胞术和 免疫过氧化物酶染色。(二)确定当地产量 不同部位炎性介质的免疫学研究 以及检测和量化这些细胞产物的生物检测方法 和核酸杂交分析，以定量 相应的mRNA。(3)确定艾滋病毒感染的影响 人单核/巨噬细胞的功能及其产生 炎性介质和长末端的部分 通过感染控制单核细胞内HIV转录的重复序列 单核细胞和单核细胞系与HIV和HIV构建。(4) 确定激活的淋巴细胞流量是否是非特异性的 与中枢神经系统中抗原的存在无关的使用 不同激活细胞群对小鼠的被动移植 通过沉淀和荧光激活的细胞分选进行纯化。 (5)确定艾滋病毒特异性病毒的存在和水平 通过检测神经组织特异性免疫反应 血清和脑脊液中存在特异性抗体。