IMMUNOLOGICAL ASPECTS OF NERVOUS SYSTEM INFECTION WITH HIV

据推测，不同的 HIV 相关神经系统 疾病有不同的发病机制。 在某些疾病中，免疫 系统预计将发挥主要作用，而在其他方面则发挥次要作用 角色，而在其他角色中则没有角色。 确定参与方 各种与艾滋病毒相关的神经系统疾病中的免疫反应 建议 (1) 识别炎症细胞及其状态 通过研究单核细胞来激活这些细胞 使用流式细胞仪检测脑脊液、血液和组织 免疫过氧化物酶染色。 (2)确定当地产量 使用免疫学方法分析不同部位的炎症介质 和生物测定来检测和定量这些细胞产物 和核酸杂交测定来定量 相应的mRNA。 (3) 确定HIV感染的影响 关于人类单核细胞/巨噬细胞的功能及其产生 炎症介质和长末端部分 通过感染重复控制单核细胞中的 HIV 转录 单核细胞和带有 HIV 和 HIV 构建体的单核细胞系。 (4) 确定活化的淋巴细胞是否非特异性运输 与中枢神经系统中抗原的存在无关的中枢神经系统使用 被动转移不同活化细胞群的小鼠 通过沉淀和荧光激活细胞分选纯化。 (5) 确定 HIV 特异性和水平的存在和水平 通过检测神经组织特异性免疫反应 血清和脑脊液中存在特异性抗体。

It is hypothesized that different HIV-associated neurological diseases have different pathogeneses. In some diseases the immune system is expected to play a primary role, in others a secondary role, and in others no role. To identify the participation of the immune response in various HIV-associated neurological diseases we propose (1) To identify the inflammatory cells and the state of activation of these cells by studying mononuclear cells in the cerebrospinal fluid, blood and tissues using flow cytometry and immunoperoxidase staining. (2) To determine the local production of inflammatory mediators in different sites using immunological and biological assays to detect and quantitate these cell products and nucleic acid hybridization assays to quantitate the corresponding mRNA. (3) To determine the effect of HIV infection on the function of human monocyte/macrophages and their production of inflammatory mediators and the portion of the long terminal repeat controlling HIV transcription in monocytes by infecting monocytes and monocyte cell lines with HIV and HIV constructs. (4) To determine whether activated lymphocytes traffic nonspecifically to the CNS independent of the presence of antigen in the CNS using passive transfer into mice of different activated cell populations purified by sedimentation and fluorescence activated cell sorting. (5) To determine the presence and level of the HIV-specific and neural tissue-specific immune responses by assaying for the presence of specific antibody in serum and CSF.