IMMUNOLOGICAL ASPECTS OF NERVOUS SYSTEM INFECTION WITH HIV

HIV 神经系统感染的免疫学方面

• 批准号: 3861560
• 负责人: D E GRIFFIN
• 金额: --
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3861560
• 关键词: HIV infections; antiviral antibody; blood; cerebrospinal fluid; cytokine; flow cytometry; histochemistry /cytochemistry; human immunodeficiency virus; inflammation; laboratory mouse; leukocyte activation /transformation; macrophage; monocyte; nervous system disorder; opportunistic infections; psychoneuroimmunology; virus antigen; virus genetics; virus replication

It is hypothesized that different HIV-associated neurological diseases have different pathogeneses. In some diseases the immune system is expected to play a primary role, in others a secondary role, and in others no role. To identify the participation of the immune response in various HIV-associated neurological diseases we propose (1) To identify the inflammatory cells and the state of activation of these cells by studying mononuclear cells in the cerebrospinal fluid, blood and tissues using flow cytometry and immunoperoxidase staining. (2) To determine the local production of inflammatory mediators in different sites using immunological and biological assays to detect and quantitate these cell products and nucleic acid hybridization assays to quantitate the corresponding mRNA. (3) To determine the effect of HIV infection on the function of human monocyte/macrophages and their production of inflammatory mediators and the portion of the long terminal repeat controlling HIV transcription in monocytes by infecting monocytes and monocyte cell lines with HIV and HIV constructs. (4) To determine whether activated lymphocytes traffic nonspecifically to the CNS independent of the presence of antigen in the CNS using passive transfer into mice of different activated cell populations purified by sedimentation and fluorescence activated cell sorting. (5) To determine the presence and level of the HIV-specific and neural tissue-specific immune responses by assaying for the presence of specific antibody in serum and CSF.

据推测，不同的艾滋病毒相关的神经系统， 疾病有不同的病因。 在某些疾病中， 在某些情况下，预期系统将发挥主要作用，在其他情况下则是次要作用。 在其他方面，没有角色。 为了确定参与者， 各种HIV相关神经系统疾病中免疫应答 建议（1）识别炎症细胞和炎症状态， 通过研究这些细胞中的单核细胞， 使用流式细胞术检测脑脊液、血液和组织， 免疫过氧化物酶染色。 (2)以确定当地生产 炎症介质在不同的网站，使用免疫 以及检测和定量这些细胞产物的生物测定 和核酸杂交测定来定量 对应的mRNA。 (3)确定艾滋病毒感染的影响 对人单核/巨噬细胞功能及其产生的影响 炎症介质和长末端的部分 通过感染来重复控制单核细胞中的HIV转录 单核细胞和单核细胞细胞系与HIV和HIV构建体。 （四） 为了确定活化的淋巴细胞是否非特异性地 不依赖于CNS中抗原的存在， 将不同活化细胞群被动转移到小鼠中 通过沉降和荧光激活细胞分选纯化。 (5)确定艾滋病毒特异性抗体的存在和水平， 神经组织特异性免疫应答， 血清和CSF中存在特异性抗体。