PHYSICS AND CHEMISTRY OF PHOTORECEPTION

光接收的物理和化学

• 批准号: 3854788
• 负责人: W A HAGINS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854788
• 关键词: Anura; bioenergetics; brain cell; calcium indicator; calorimetry; cell water; electron probe spectrometry; fluorescent dye /probe; high performance liquid chromatography; mass spectrometry; membrane potentials; photochemistry; photostimulus; retina; thermodynamics; visual photoreceptor

Improvements and extensions of pyroelectric calorimetry for study of heat production by milligram weight biochemical and cellular systems are under development. Pyroelectric calorimetric studies of phototransduction in frog retinas are being extended to low light exposures and to retinas that have been subjected to sudden changes in their fluid environment. A series of new compounds have been synthesized to be useful as elemental standards for estimating water content and transmembrane potentials in retinal and brain cells. Distributions of these compounds is being studies by electron energy-loss spectrometry. The metabolism of esterified membrane-permeable fluorescent calcium indicator dyes is being studied by high-pressure liquid chromatography and mass spectrometry, with the aim of explaining the uneven cellular distribution of the dyes and the presence of fluorescent but calcium- insensitive forms in retinal photoreceptors.

热释电量热法的改进和扩展 由毫克重量生物化学和细胞系统产生热量， 正在开发中。 蛙视网膜光传导的热释电量热研究 被扩展到低光暴露和视网膜， 在流体环境中受到突然的变化。 已经合成了一系列新的化合物， 用于估计水含量和跨膜的元素标准 视网膜和脑细胞中的电位。这些化合物的分布 正在用电子能量损失谱法进行研究。 酯化透膜荧光钙的代谢 指示染料正在研究高压液相色谱法 和质谱分析，目的是解释不均匀的细胞 染料的分布和荧光的存在，但钙- 视网膜感光器中的不敏感形式。