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SITE-DIRECTED MUTAGENESIS OF NONMUSCLE MYOSIN HEAVY CHAINS

SITE-DIRECTED MUTAGENESIS OF NONMUSCLE MYOSIN HEAVY CHAINS
非肌肉肌球蛋白重链的定点诱变
批准号：
3858105
负责人：
R S ADELSLEIN
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3858105
关键词：
Insecta animal tissue brain chickens complementary DNA gene expression genetic library genetic manipulation molecular weight myosins protein sequence protein structure function site directed mutagenesis transfection transposon /insertion element

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英文摘要
3.7 kbp of cDNA previously found to encode the head region of chicken brain nonmuscle myosin heavy chain (a B isoform of vertebrate nonmuscle myosin II heavy chain) was inserted into the insect vector pBlueBac and will be transfected into insect cells with the purpose of expressing mg quantities of the myosin heavy chain. Our goal is to recover a fully active nonmuscle myosin heavy chain so that we can examine the role of the myosin light chains on enzymatic activity as well as the effect of the inserted amino acid sequences we have recently discovered in the head portion of myosin cDNA cloned from a chicken brain library.
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SITE-DIRECTED MUTAGENESIS OF NONMUSCLE MYOSIN HEAVY CHAINS
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