FINNEGAN MATT SSQ-700 WITH ADDITIONAL DATA SYSTEM

FINNEGAN MATT SSQ-700 带附加数据系统

• 批准号: 3521036
• 负责人: WALTER C HUBBARD
• 金额: $ 14.9万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-22 至 1993-05-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521036
• 关键词: biomedical equipment purchase; biomedical facility; eicosanoids; hypersensitivity; mass spectrometry; prostaglandins

Combined capillary gas chromatography-negative ion chemical ionization mass spectrometry provid and quantitation of low-levels of prostaglandins, leukotrienes, platelet-activating factor (PAF), hydroxy-eicosanoids, keto-eicosanoids and diacylglycerides in complex biological fluids. Funds are requested for the purchase of a Finnigan MAT SSQ-700R single stage quadrupole GC/MS/DS with additional data system 8-Mb RAM expansion and two buck/boost transformers (acquisition cost - $264,700) to be shared by ten co-investigators. The instrumentation will be used to provide dedicated instrumentation for combined capillary gas chromatography-negative ion chemical ionization mass spectrometric techniques critical for determination of the role of lipid mediators in human asthma, allergy, pulmonary disease and pulmonary injury. The prostaglandins, leukotriene B4, hydroxy-eicosanoids, keto-eicosanoids and the diacylglycerides are directly converted to electron-capture derivatives with suitable vapor phase properties via facile reaction with multifluorinated reagents. The sulfidopeptido- leukotrienes (leukotrienes C4, D4 and E4) and PAF are chemically modified prior to synthesis of electron-capture derivatives. The sulfidopeptido group of leukotrienes C4, D4 and E4 is removed leaving an eicosanoid skeleton which is then converted to the pentafluorobenzyl ester. The phosphate ester of PAF is hydrolyzed prior to synthesis of the diglyceride pentafluorobenzoyl ester derivative. The exquisite sensitivity (detection limits < 100 fg/injection) of combined capillary gas chromatography-negative ion chemical ionization mass spectrometry has resulted in the adaptation of simplified and less labor-intensive purification procedures than previously required in mass spectrometric assays. In turn, more complete determinations of the profiles of lipid mediators in biological samples are obtainable. The proposed instrumentation should provide information for more rational approaches in the prevention, management and treatment of asthma, allergy, pulmonary disease and pulmonary injury.

毛细管气相色谱-负离子化学电离联用 质谱法提供了低水平 三尖杉酯碱，白三烯，血小板活化因子（PAF）， 羟基类二十烷酸、酮基类二十烷酸和甘油二酯复合物 生物液体 请求提供资金，用于购买一辆 MAT SSQ-700 R单级四极杆GC/MS/DS 系统8 Mb RAM扩展和两个降压/升压变压器（获取 费用-264 700美元），由10名共同调查员分担。 的 仪器将用于提供专用仪器， 毛细管气相色谱-负离子化学电离联用 质谱技术对于确定 人类哮喘、变态反应、肺部疾病和 肺损伤 这些化合物包括白藜芦醇，白三烯B4，羟基类二十烷酸， 酮-类二十烷酸和二酰基甘油酯直接转化为 具有合适的气相性质的电子捕获衍生物， 与多氟化试剂反应容易。 硫肽- 白三烯（白三烯C4、D4和E4）和PAF是化学上 在合成电子捕获衍生物之前进行修饰。 的 去除白三烯C4、D4和E4的硫代肽基， 类二十烷骨架，然后转化为五氟苄基 酯。 PAF的磷酸酯在合成前水解， 甘油二酯五氟苯甲酰基酯衍生物。 的精致 组合毛细管的灵敏度（检测限< 100 fg/进样） 气相色谱-负离子化学电离质谱 已经导致了简化和劳动密集度较低的适应 比以前质谱分析中所需的纯化程序 测定。 反过来，更完整的测定血脂谱， 生物样品中的介体是可获得的。 拟议 仪表应提供信息，以更合理的方法 在预防、管理和治疗哮喘，过敏， 肺疾病和肺损伤。