Micromass Quattro API TSQ For LC-MS/MS Quantitation

用于 LC-MS/MS 定量的 Micromass Quattro API TSQ

• 批准号: 6440264
• 负责人: WALTER C HUBBARD
• 金额: $ 34.04万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440264
• 关键词: biomedical equipment purchase; hypersensitivity; inflammation; liquid chromatography mass spectrometry; respiratory disorder

DESCRIPTION (provided by applicant): Eight established investigators require robust state-of-the-art LC-MS instrumentation dedicated to low level quantitation of phospholipids and products of phospholipid metabolism, fatty acid acyl conjugates of hydrocortisone and triamcinolone acetonide (TAA), neuropeptides, endogenous levels of hydrocortisone and cortisone in nasal and bronchoalveolar lavage fluids and plasma, sphingosine-l-phosphate (SIP) and peptidoleukotrienes in samples originating from laboratory and clinical studies. Funding is requested for one Micromass Quattro Ultima LC Benchtop Triple Stage Quadrupole Mass Spectrometer (acquisition cost = $340,420) for installation in the laboratory dedicated as a core analytical facility for the investigators' needs. Quantitative LC-MS techniques will provide critical data for defining the role of phospholipids and sphingolipids in initiation of cell signaling, modulation of cell activation, calcium release, signal transduction and other cellular events resulting cell activation that occur in allergic reactions, asthma, inflammation, vascular injury and pulmonary obstruction. The proposed instrumentation will also greatly facilitate better understanding of the neuronal component (s) of asthma and pulmonary disease by providing critical data related to the extent of neuropeptide release in normal, inflamed and asthmatic airways and lung tissue. Studies of the metabolism of hydrocortisone and TAA in lung tissue and the airways may define a modulatory role of hydrocortisone and anti-inflammatory effects of TAA via mechanisms not previously recognized. In addition, the instrumentation will provide a highly selective analytical technique for verification of high throughput assay methods used for measurement of peptidoleukotriene release by basophils in cell suspensions and by basophils and mast cells in the airways. The quantitative data provided by the proposed instrumentation will provide information for more rational approaches for the prevention, management and treatment of asthma, allergic and inflammatory reactions, adult respiratory distress syndrome and obstructive pulmonary disease.

描述(由申请者提供)：八名资深调查人员需要 坚固的最先进的LC-MS仪器专用于低电平 脂肪磷脂及磷脂代谢产物的定量 氢化可的松和曲安奈德(TAA)的酸性酰基偶合物， 神经肽、内源性氢化可的松和可的松在鼻腔和鼻腔的含量 支气管肺泡灌洗液和血浆、鞘氨醇-L-磷酸和 实验室和临床样品中的多肽优三烯 学习。为一台微质量Quattro Ultima LC台面申请资金 三级四极质谱计(购置成本=340,420美元) 在实验室中安装，作为核心分析设备 调查人员的需求。定量LC-MS技术将提供关键数据 用于确定磷脂和鞘脂在细胞启动中的作用 信号、细胞激活的调节、钙释放、信号转导 以及其他导致细胞激活的细胞事件发生在过敏症中 反应、哮喘、炎症、血管损伤和肺梗阻。这个 建议的工具也将大大有助于更好地理解 哮喘和肺部疾病的神经元成分(S)通过提供 与正常、炎症时神经肽释放程度相关的关键数据 哮喘的呼吸道和肺组织。人血清白蛋白代谢研究 肺组织和呼吸道中的氢化可的松和TAA可能定义了一种 氢化可的松的作用及TAA的抗炎作用 以前认识到的。此外，该仪器将提供高度的 用于验证高通量分析的选择性分析技术 细胞内嗜碱性粒细胞释放肽的测定方法 悬浮和呼吸道中的嗜碱性粒细胞和肥大细胞。量化的 建议的工具提供的数据将提供更多信息 合理预防、管理和治疗哮喘， 过敏和炎症反应，成人呼吸窘迫综合征和 阻塞性肺病。

项目成果

Accelerator mass spectrometry measurement of intracellular concentrations of active drug metabolites in human target cells in vivo.

加速器质谱法测量人体靶细胞体内活性药物代谢物的细胞内浓度。

• DOI: 10.1038/clpt.2010.188
• 发表时间: 2010
• 期刊: Clinical pharmacology and therapeutics
• 影响因子: 6.7
• 作者: Chen,J;Garner,RC;Lee,LS;Seymour,M;Fuchs,EJ;Hubbard,WC;Parsons,TL;Pakes,GE;Fletcher,CV;Flexner,C
• 通讯作者: Flexner,C