Applied Biosystems API5500 Triple Quadrupole Mass Spectrometer

Applied Biosystems API5500 三重四极杆质谱仪

• 批准号: 7795638
• 负责人: WALTER C HUBBARD
• 金额: $ 35.04万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795638
• 关键词: AIDS/HIV problem; African Trypanosomiasis; Anti-Infective Agents; Arts; Atmospheric Pressure; Back; Biological Assay; Cause of Death; Chemicals; Clinical; Clinical Trials; Communicable Diseases; Country; Data; Detection; Disease; Drug Kinetics; Economic Burden; Electrospray Ionization; Epidemic; Event; Failure; Fatal Outcome; Funding; HIV; Health; Housing; International; International Health Problems; Laboratory Study; Malaria; Metabolism; Morbidity - disease rate; Population; Prevalence; Prevention; Productivity; Qualifying; Research; Research Personnel; Resources; Sampling; Scientist; System; Techniques; Therapeutic; Trypanosomiasis; Tuberculosis; Vaccines; Water; Work; assay development; burden of illness; cost; falls; instrument; instrumentation; ionization; mass spectrometer; mortality; multiple reaction monitoring; novel therapeutic intervention; novel therapeutics; pandemic disease; prevent; public health relevance

DESCRIPTION (provided by applicant): HIV/AIDS, tuberculosis (TB), malaria and trypanosomiasis are infectious diseases of major importance to international health causing tremendous disease burdens on populations globally. HIV, the greatest cause of death internationally, is pandemic with fatal outcomes and dim prospects for vaccine prevention. TB, the second greatest cause of death, exists throughout the world and is epidemic in many regions where its prevalence is great and its mortality is common. Malaria and trypanosomiasis cause immense hardship in some of the world's most resource-limited countries. In this proposal seven established and qualifying NIH-funded investigators are joined by 5 other funded investigators to focus research efforts on the treatment or prevention of these 4 major international health problems. To do so, we require robust state-of-the-art LC-MS/MS instrumentation dedicated to detection and quantitation of low picomolar to femtomolar levels of therapeutic anti-infectives. Funding is requested for purchase of one Applied Biosystems API5500 LC-MS/MS system with a Water Acquity UPLC interface with capabilities for electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). The addition of this mass spectrometer system will provide adequate access to qualified investigators that currently have either extremely limited or no access to acceptable instrumentation or currently have assay commitments that exceed their capacity. The acquisition cost is $350,350; institutional support for its proper housing and productive use is robust. Quantitative LC-MS/MS multiple reaction monitoring (MRM) techniques will provide critical data related to therapeutic levels, pharmacokinetics and metabolism of anti-infectives employed or being evaluated for these diseases. The availability of the requested instrumentation will provide a substantial increase in sample throughput capacity, will support new research initiatives, will allow the seamless transition from assay development to sample analysis on more than one instrument, and will provide a back-up mechanism that prevents loss of precious clinical samples in the event of an instrument failure. Despite the morbidity, mortality and economic burden of these diseases, which are widely recognized to be enormous, new therapeutic advances often fall to NIH-sponsored academic scientists. The availability of the requested instrument would substantially increase the productivity of these investigators at Johns Hopkins. PUBLIC HEALTH RELEVANCE: The requested API5500 mass spectrometer is needed to support the NIH-funded research of scientists who are working to develop new preventions and treatments for HIV, tuberculosis, malaria and African trypanosomiasis. The availability of this instrument will greatly increase their ability to study new therapeutic approaches in laboratory studies and in clinical trials.

描述（由申请人提供）：艾滋病、结核病、疟疾和锥虫病是对国际卫生具有重要意义的传染病，对全球人口造成巨大的疾病负担。艾滋病毒是国际上最大的死亡原因，是一种具有致命后果和疫苗预防前景黯淡的流行病。结核病是导致死亡的第二大原因，在世界各地都存在，在许多流行率高、死亡率普遍的地区流行。疟疾和锥虫病在世界上一些资源最有限的国家造成了巨大的困难。在这项提案中，7名已获得美国国立卫生研究院资助的合格研究人员与另外5名受资助的研究人员一起将研究重点放在这4个主要国际健康问题的治疗或预防上。为此，我们需要强大的最先进的LC-MS/MS仪器，专门用于检测和定量低皮摩尔至飞摩尔水平的治疗性抗感染药物。申请资金用于购买一台应用生物系统API5500 LC-MS/MS系统，该系统具有水处理UPLC接口，具有电喷雾电离（ESI）和大气压化学电离（APCI）功能。这种质谱仪系统的增加将为合格的研究人员提供足够的机会，这些研究人员目前要么非常有限，要么无法获得可接受的仪器，要么目前的分析承诺超出了他们的能力。收购成本为350,350美元；对其适当住房和生产使用的体制支持是强有力的。定量LC-MS/MS多反应监测（MRM）技术将为这些疾病的抗感染药物的治疗水平、药代动力学和代谢提供关键数据。所需仪器的可用性将大大提高样品吞吐量，将支持新的研究计划，将允许从检测开发到使用多台仪器进行样品分析的无缝过渡，并将提供备份机制，防止在仪器故障时丢失宝贵的临床样品。尽管这些疾病的发病率、死亡率和经济负担都被广泛认为是巨大的，但新的治疗进展往往落在美国国立卫生研究院资助的学术科学家身上。所要求的仪器的可用性将大大提高这些研究人员在约翰霍普金斯大学的生产力。