MOLECULAR BIOLOGY OF OUTER RETINA-SPECIFIC PROTEINS

视网膜色素上皮(RPE)细胞和感光细胞(PR)是 在功能上和发展上紧密结合。在开发过程中 大鼠的外视网膜，RPE特异性识别的决定因素 单抗RPE-9在出生后第3天首次表达，而 PR外层部分(OS)在第5天出现。OS首先出现在RPE的位置 细胞已经表达了它们的决定因素。RPE-9识别67 kDa RPE特有的蛋白质。这种蛋白质存在于哺乳动物和禽类中。 RPE。作为一种膜相关蛋白，它可能是非糖基化的。我们 已经开始在牛RPE的cDNA文库中筛选该蛋白的cDNA. 我们已经亚克隆了对应于前两个重复序列的DNA片段 将牛IRBP基因克隆入细菌表达载体。IRBP参与了 维甲酸的运输，RPE和RPE之间的功能关系 公共关系由此产生的表达蛋白质片段将被测试其 配体结合和免疫学性质。

Retinal pigment epithelial (RPE) cells and the photoreceptor (PR) cells are functionally and developmentally closely integrated. During development of the outer retina in rat, the determinant recognized by the RPE-specific monoclonal antibody RPE-9 is first expressed at post-natal day 3 whereas the PR outer segments (OS) appear at day 5. The OS first appears where RPE cells are already expressing their determinant. RPE-9 recognizes a 67-kDa protein specific to the RPE. This protein is found in mammalian and avian RPE. A membrane-associated protein, it is probably non-glycosylated. We have begun to screen a bovine RPE cDNA library for a cDNA for this protein. We have subcloned DNA fragments corresponding to the first two repeats of bovine IRBP into a bacterial expression vector. IRBP is involved in the transport of retinoids, a functional relationship between the RPE and the PR. The resultant expressed protein fragments will be tested for their ligand-binding and immunological properties.