MOLECULAR BIOLOGY OF OUTER RETINA-SPECIFIC PROTEINS

视网膜色素上皮（RPE）细胞和光感受器（PR）细胞是 功能和发展紧密结合。开发过程中 大鼠视网膜外层，RPE特异性识别的决定子 单克隆抗体RPE-9在出生后第3天首次表达， PR外段（OS）在第5天出现。操作系统首先出现在RPE 细胞已经表达了它们的决定簇。RPE-9识别67-kDa的 RPE特异性蛋白质。这种蛋白质存在于哺乳动物和鸟类中 RPE。作为一种膜相关蛋白，它可能是非糖基化的。我们 已经开始在牛RPE cDNA文库中筛选这种蛋白的cDNA。 我们已经亚克隆了对应于前两个重复序列的DNA片段， 将牛IRBP导入细菌表达载体。IRBP参与了 类维生素A的运输，RPE和视网膜色素上皮细胞之间的功能关系， PR.将测试所得表达的蛋白片段的 配体结合和免疫学特性。

Retinal pigment epithelial (RPE) cells and the photoreceptor (PR) cells are functionally and developmentally closely integrated. During development of the outer retina in rat, the determinant recognized by the RPE-specific monoclonal antibody RPE-9 is first expressed at post-natal day 3 whereas the PR outer segments (OS) appear at day 5. The OS first appears where RPE cells are already expressing their determinant. RPE-9 recognizes a 67-kDa protein specific to the RPE. This protein is found in mammalian and avian RPE. A membrane-associated protein, it is probably non-glycosylated. We have begun to screen a bovine RPE cDNA library for a cDNA for this protein. We have subcloned DNA fragments corresponding to the first two repeats of bovine IRBP into a bacterial expression vector. IRBP is involved in the transport of retinoids, a functional relationship between the RPE and the PR. The resultant expressed protein fragments will be tested for their ligand-binding and immunological properties.